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TitleEngineering the PP7 Virus Capsid as a Peptide Display Platform.
Journal, issue, pagesACS Nano, Vol. 13, Issue 4, Page 4443-4454, Year 2019
Publish dateApr 23, 2019
AuthorsLiangjun Zhao / Mykhailo Kopylov / Clinton S Potter / Bridget Carragher / M G Finn /
PubMed AbstractAs self-assembling polyvalent nanoscale structures that can tolerate substantial genetic and chemical modification, virus-like particles are useful in a variety of fields. Here we describe the ...As self-assembling polyvalent nanoscale structures that can tolerate substantial genetic and chemical modification, virus-like particles are useful in a variety of fields. Here we describe the genetic modification and structural characterization of the Leviviridae PP7 capsid protein as a platform for the presentation of functional polypeptides. This particle was shown to tolerate the display of sequences from 1 kDa (a cell penetrating peptide) to 14 kDa (the Fc-binding double Z-domain) on its exterior surface as C-terminal genetic fusions to the coat protein. In addition, a dimeric construct allowed the presentation of exogenous loops between capsid monomers and the simultaneous presentation of two different peptides at different positions on the icosahedral structure. The PP7 particle is thereby significantly more tolerant of these types of polypeptide additions than Qβ and MS2, the other Leviviridae-derived VLPs in common use.
External linksACS Nano / PubMed:30912918 / PubMed Central
MethodsEM (single particle)
Resolution2.89 - 3.43 Å
Structure data

0344

6n4v

EMDB-0344, PDB-6n4v:
CryoEM structure of Leviviridae PP7 WT coat protein dimer capsid (PP7PP7-WT)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-0351:
CryoEM structure of Leviviridae PP7 WT coat protein dimer capsid (PP7PP7-WT)
Method: EM (single particle) / Resolution: 3.32 Å

EMDB-0352:
CryoEM structure of Leviviridae PP7 WT coat protein dimer capsid (PP7PP7-WT)
Method: EM (single particle) / Resolution: 3.21 Å

EMDB-0353:
CryoEM structure of Leviviridae PP7 coat protein dimer capsid with a loop insertion (PP7-aloop-PP7)
Method: EM (single particle) / Resolution: 2.89 Å

EMDB-0354:
CryoEM structure of Leviviridae PP7 coat protein dimer capsid with a loop insertion and a C-terminal extension (PP7-aloop-PP7-150loop)
Method: EM (single particle) / Resolution: 3.43 Å

Source
  • pseudomonas phage pp7 (virus)
KeywordsVIRUS LIKE PARTICLE / T4 / icosahedral / PP7 / biotechnology / vaccine / drug delivery

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