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Structure paper

TitleN-glycan Utilization by Bifidobacterium Gut Symbionts Involves a Specialist beta-Mannosidase.
Journal, issue, pagesJ. Mol. Biol., Vol. 431, Page 732-747, Year 2019
Publish dateOct 5, 2018 (structure data deposition date)
AuthorsCordeiro, R.L. / Pirolla, R.A.S. / Persinoti, G.F. / Gozzo, F.C. / de Giuseppe, P.O. / Murakami, M.T.
External linksJ. Mol. Biol. / PubMed:30641082
MethodsX-ray diffraction
Resolution1.75 - 2.5 Å
Structure data

PDB-6moy:
Crystal structure of the E257A mutant of BlMan5B in complex with GlcNAc (co-crystallization)
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-6mp2:
Crystal structure of BlMan5B solved by SIRAS
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

PDB-6mp7:
Crystal structure of the E257A mutant of BlMan5B in complex with GlcNAc (soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-6mpa:
Crystal structure of BlMan5B in complex with GlcNAc (soaking)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6mpc:
Crystal structure of E257A mutant of BlMan5B
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-FLC:
CITRATE ANION

ChemComp-NA:
Unknown entry

Source
  • bifidobacterium longum (strain djo10a) (bacteria)
  • bifidobacterium longum djo10a (bacteria)
KeywordsHYDROLASE / Family GH5 / subfamily 18 / beta-mannosidase

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