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-Structure paper
タイトル | Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex. |
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ジャーナル・号・ページ | PLoS Biol, Vol. 16, Issue 11, Page e3000050, Year 2018 |
掲載日 | 2018年11月9日 |
著者 | Zhuan Qin / Jiagang Tu / Tao Lin / Steven J Norris / Chunhao Li / Md A Motaleb / Jun Liu / |
PubMed 要旨 | Periplasmic flagella are essential for the distinct morphology and motility of spirochetes. A flagella-specific type III secretion system (fT3SS) composed of a membrane-bound export apparatus and a ...Periplasmic flagella are essential for the distinct morphology and motility of spirochetes. A flagella-specific type III secretion system (fT3SS) composed of a membrane-bound export apparatus and a cytosolic ATPase complex is responsible for the assembly of the periplasmic flagella. Here, we deployed cryo-electron tomography (cryo-ET) to visualize the fT3SS machine in the Lyme disease spirochete Borrelia burgdorferi. We show, for the first time, that the cytosolic ATPase complex is attached to the flagellar C-ring through multiple spokes to form the "spoke and hub" structure in B. burgdorferi. This structure not only strengthens structural rigidity of the round-shaped C-ring but also appears to rotate with the C-ring. Our studies provide structural insights into the unique mechanisms underlying assembly and rotation of the periplasmic flagella and may provide the basis for the development of novel therapeutic strategies against several pathogenic spirochetes. |
リンク | PLoS Biol / PubMed:30412577 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 29.0 - 33.0 Å |
構造データ | EMDB-9122: EMDB-9123: |
由来 |
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