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Structure paper

TitleStructural basis for murine norovirus engagement of bile acids and the CD300lf receptor.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 115, Issue 39, Page E9201-E9210, Year 2018
Publish dateSep 25, 2018
AuthorsChristopher A Nelson / Craig B Wilen / Ya-Nan Dai / Robert C Orchard / Arthur S Kim / Roderick A Stegeman / Leon L Hsieh / Thomas J Smith / Herbert W Virgin / Daved H Fremont /
PubMed AbstractMurine norovirus (MNoV) is closely related to human norovirus (HNoV), an infectious agent responsible for acute gastroenteritis worldwide. Here we report the X-ray crystal structure of the dimeric ...Murine norovirus (MNoV) is closely related to human norovirus (HNoV), an infectious agent responsible for acute gastroenteritis worldwide. Here we report the X-ray crystal structure of the dimeric MNoV VP1 protruding (P) domain in complex with its cellular receptor CD300lf. CD300lf binds the P domain with a 2:2 stoichiometry, engaging a cleft between the AB and DE loops of the P2 subdomain at a site that overlaps the epitopes of neutralizing antibodies. We also identify that bile acids are cofactors enhancing MNoV cell-binding and infectivity. Structures of CD300lf-P domain in complex with glycochenodeoxycholic acid (GCDCA) and lithocholic acid (LCA) reveal two bile acid binding sites at the P domain dimer interface distant from receptor binding sites. The structural determinants for receptor and bile acid binding are supported by numerous biophysical assays utilizing interface residue mutations. We find that the monomeric affinity of CD300lf for the P domain is low and is divalent cation dependent. We have also determined the crystal structure of CD300lf in complex with phosphocholine, revealing that MNoV engages its receptor in a manner mimicking host ligands including similar metal coordination. Docking of the cocomplex structures onto a cryo-EM-derived model of MNoV suggests that each virion can make multiple CD300lf engagements, and thus, infection may be driven by the avidity of cell surface clustered CD300lf. These studies identify multiple potential modulators of norovirus infection that may act to regulate the interaction between the viral capsid P domain and its cognate cellular receptor.
External linksProc Natl Acad Sci U S A / PubMed:30194229 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.358 - 8.0 Å
Structure data

7564

6crj

EMDB-7564, PDB-6crj:
Mouse norovirus model using the crystal structure of MNV P domain and the Norwalkvirus shell domain
Method: EM (single particle) / Resolution: 8.0 Å

PDB-6c6q:
Crystal Structure of the Murine Norovirus VP1 P Domain in complex with the CD300lf Receptor
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-6c74:
Crystal Structure of Murine CD300lf in complex with phosphocholine
Method: X-RAY DIFFRACTION / Resolution: 1.358 Å

PDB-6e47:
Crystal Structure of the Murine Norovirus VP1 P domain in complex with the CD300lf Receptor and Glycochenodeoxycholic Acid
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-6e48:
Crystal Structure of the Murine Norovirus VP1 P domain in complex with the CD300lf Receptor and Lithocholic Acid
Method: X-RAY DIFFRACTION / Resolution: 1.801 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-CL:
Unknown entry / Chloride

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

ChemComp-PC:
PHOSPHOCHOLINE / Phosphocholine

ChemComp-CA:
Unknown entry

ChemComp-CHO:
GLYCOCHENODEOXYCHOLIC ACID / detergent*YM / Glycochenodeoxycholic acid

ChemComp-4OA:
(3beta,5beta,14beta,17alpha)-3-hydroxycholan-24-oic acid / detergent*YM / Lithocholic acid

Source
  • murine norovirus 1
  • mus musculus (house mouse)
  • norwalk virus
KeywordsVIRAL PROTEIN/LIPID BINDING PROTEIN / Norovirus / Capsid protein / Protruding domain / Ig-V like / VIRAL PROTEIN-LIPID BINDING PROTEIN complex / CD300lf / VP1 / myeloid receptor / CMRF-35-like molecule-1 / CLM-1 / CLM1 / DIR2 / IREM1 / LMIR3 / PIGR3 / IgSF13 / LIPID BINDING PROTEIN / IgV-like domain / PIGR3. IgSF13 / VIRUS / mouse / VIRAL PROTEIN / bile acid / GCDCA / LCA

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