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Structure paper

TitleStructural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase fromSalmonella enterica.
Journal, issue, pagesJ. Biol. Chem., Vol. 293, Page 11481-11490, Year 2018
Publish dateMay 7, 2016 (structure data deposition date)
AuthorsStern, A.L. / Van der Verren, S.E. / Kanchugal P, S. / Nasvall, J. / Gutierrez-de-Teran, H. / Selmer, M.
External linksJ. Biol. Chem. / PubMed:29871922
MethodsX-ray diffraction
Resolution1.4 - 2.05 Å
Structure data

PDB-5g4a:
AadA in complex with ATP and magnesium
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5lpa:
AadA E87Q in complex with ATP, calcium and dihydrostreptomycin
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-5luh:
AadA E87Q in complex with ATP, calcium and streptomycin
Method: X-RAY DIFFRACTION / Resolution: 1.73 Å

PDB-6fzb:
AadA in complex with ATP, magnesium and streptomycin
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-CL:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-UNX:
Unknown entry

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HOH:
WATER

ChemComp-71R:
dihydrostreptomycin / antibiotic*YM

ChemComp-CA:
Unknown entry

ChemComp-SRY:
STREPTOMYCIN / medication, antibiotic*YM

ChemComp-GOL:
GLYCEROL

Source
  • salmonella enterica (bacteria)
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
  • salmonella enterica subsp. enterica serovar typhimurium str. lt2 (bacteria)
KeywordsTRANSFERASE / AMINOGLYCOSIDE ADENYL TRANSFERASE / ANT(3'')9 / ANTIBIOTIC RESISTANCE

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