EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Ligand binding and conformational changes of SUR1 subunit in pancreatic ATP-sensitive potassium channels.
ジャーナル・号・ページ	Protein Cell, Vol. 9, Issue 6, Page 553-567, Year 2018
掲載日	2018年3月28日
著者	Jing-Xiang Wu / Dian Ding / Mengmeng Wang / Yunlu Kang / Xin Zeng / Lei Chen /
PubMed 要旨	ATP-sensitive potassium channels (K) are energy sensors on the plasma membrane. By sensing the intracellular ADP/ATP ratio of β-cells, pancreatic K channels control insulin release and regulate ...ATP-sensitive potassium channels (K) are energy sensors on the plasma membrane. By sensing the intracellular ADP/ATP ratio of β-cells, pancreatic K channels control insulin release and regulate metabolism at the whole body level. They are implicated in many metabolic disorders and diseases and are therefore important drug targets. Here, we present three structures of pancreatic K channels solved by cryo-electron microscopy (cryo-EM), at resolutions ranging from 4.1 to 4.5 Å. These structures depict the binding site of the antidiabetic drug glibenclamide, indicate how Kir6.2 (inward-rectifying potassium channel 6.2) N-terminus participates in the coupling between the peripheral SUR1 (sulfonylurea receptor 1) subunit and the central Kir6.2 channel, reveal the binding mode of activating nucleotides, and suggest the mechanism of how Mg-ADP binding on nucleotide binding domains (NBDs) drives a conformational change of the SUR1 subunit.
リンク	Protein Cell / PubMed:29594720 / PubMed Central
手法	EM (単粒子)
解像度	4.11 - 6.1 Å
構造データ	6831 5yke EMDB-6831, PDB-5yke: Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (focused refinement on TM at 4.11A) 手法: EM (単粒子) / 解像度: 4.11 Å 6832 5ykf EMDB-6832, PDB-5ykf: Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (3D class1 at 4.33A) 手法: EM (単粒子) / 解像度: 4.33 Å 6833 5ykg EMDB-6833, PDB-5ykg: Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (Class2 at 4.57A) 手法: EM (単粒子) / 解像度: 4.57 Å 6847 5yw7 EMDB-6847, PDB-5yw7: Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (focused refinement on SUR1 ABC transporter module at 4.4A) 手法: EM (単粒子) / 解像度: 4.4 Å 6848 5yw8 EMDB-6848, PDB-5yw8: Structure of pancreatic ATP-sensitive potassium channel bound with ATPgammaS (all particles at 4.4A) 手法: EM (単粒子) / 解像度: 4.4 Å 6849 5yw9 EMDB-6849, PDB-5yw9: Structure of pancreatic ATP-sensitive potassium channel bound with ATPgammaS (class1 5.0A) 手法: EM (単粒子) / 解像度: 5.0 Å 6850 5ywa EMDB-6850, PDB-5ywa: Structure of pancreatic ATP-sensitive potassium channel bound with ATPgammaS (CTD class 2 at 6.1A) 手法: EM (単粒子) / 解像度: 6.1 Å 6851 5ywb EMDB-6851, PDB-5ywb: Structure of pancreatic ATP-sensitive potassium channel bound with Mg-ADP (CTD class2 at 5.2A) 手法: EM (単粒子) / 解像度: 5.2 Å 6852 5ywc EMDB-6852, PDB-5ywc: Structure of pancreatic ATP-sensitive potassium channel bound with Mg-ADP (CTD class1 at 4.3A) 手法: EM (単粒子) / 解像度: 4.3 Å 6853 5ywd EMDB-6853, PDB-5ywd: Structure of pancreatic ATP-sensitive potassium channel bound with Mg-ADP (focused refinement of SUR1 ABC transporter module at 4.22A) 手法: EM (単粒子) / 解像度: 4.2 Å
化合物	ChemComp-GBM: 5-chloro-N-(2-{4-[(cyclohexylcarbamoyl)sulfamoyl]phenyl}ethyl)-2-methoxybenzamide / グリベンクラミド / 薬剤YM ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	mus musculus (ハツカネズミ) mesocricetus auratus (ネズミ)
キーワード	MEMBRANE PROTEIN / KATP / channel / glibenclamide / sulfonylurea