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TitleTuning a Protein-Labeling Reaction to Achieve Highly Site Selective Lysine Conjugation.
Journal, issue, pagesChembiochem, Vol. 19, Page 799-804, Year 2018
Publish dateOct 31, 2017 (structure data deposition date)
AuthorsPham, G.H. / Ou, W. / Bursulaya, B. / DiDonato, M. / Herath, A. / Jin, Y. / Hao, X. / Loren, J. / Spraggon, G. / Brock, A. ...Pham, G.H. / Ou, W. / Bursulaya, B. / DiDonato, M. / Herath, A. / Jin, Y. / Hao, X. / Loren, J. / Spraggon, G. / Brock, A. / Uno, T. / Geierstanger, B.H. / Cellitti, S.E.
External linksChembiochem / PubMed:29388367
MethodsX-ray diffraction
Resolution1.75 - 2.057 Å
Structure data

PDB-6bhz:
Trastuzumab Fab D185A (Light Chain) Mutant.
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-6bi0:
Trastuzumab Fab N158A, D185A, K190A (Light Chain) Triple Mutant.
Method: X-RAY DIFFRACTION / Resolution: 2.057 Å

PDB-6bi2:
Trastuzumab Fab D185A (Light Chain) Mutant Biotin Conjugation.
Method: X-RAY DIFFRACTION / Resolution: 1.801 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-BTN:
BIOTIN

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / Fab / immunoglobulin / mutant

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