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TitleCrystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit C.
Journal, issue, pagesSci Rep, Vol. 7, Issue 1, Page 13018, Year 2017
Publish dateOct 12, 2017
AuthorsShingo Nojima / Ayumi Fujishima / Koji Kato / Kayoko Ohuchi / Nobutaka Shimizu / Kento Yonezawa / Kenji Tajima / Min Yao /
PubMed AbstractBacterial cellulose (BC) is synthesized and exported through the cell membrane via a large protein complex (terminal complex) that consists of three or four subunits. BcsC is a little-studied subunit ...Bacterial cellulose (BC) is synthesized and exported through the cell membrane via a large protein complex (terminal complex) that consists of three or four subunits. BcsC is a little-studied subunit considered to export BC to the extracellular matrix. It is predicted to have two domains: a tetratrico peptide repeat (TPR) domain and a β-barrelled outer membrane domain. Here we report the crystal structure of the N-terminal part of BcsC-TPR domain (Asp24-Arg272) derived from Enterobacter CJF-002. Unlike most TPR-containing proteins which have continuous TPR motifs, this structure has an extra α-helix between two clusters of TPR motifs. Five independent molecules in the crystal had three different conformations that varied at the hinge of the inserted α-helix. Such structural feature indicates that the inserted α-helix confers flexibility to the chain and changes the direction of the TPR super-helix, which was also suggested by structural analysis of BcsC-TPR (Asp24-Leu664) in solution by size exclusion chromatography-small-angle X-ray scattering. The flexibility at the α-helical hinge may play important role for exporting glucan chains.
External linksSci Rep / PubMed:29026093 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution3.272 Å
Structure data

SASDD53:
Tetratrico peptide repeat domain of Bacterial cellulose synthesis subunit C
Method: SAXS/SANS

PDB-5xw7:
Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthase subunit C
Method: X-RAY DIFFRACTION / Resolution: 3.272 Å

Source
  • enterobacter sp. cjf-002 (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / cellulose synthase / tetratrico peptide repeat

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