+検索条件
-Structure paper
タイトル | A structural model for microtubule minus-end recognition and protection by CAMSAP proteins. |
---|---|
ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 24, Issue 11, Page 931-943, Year 2017 |
掲載日 | 2017年10月9日 |
![]() | Joseph Atherton / Kai Jiang / Marcel M Stangier / Yanzhang Luo / Shasha Hua / Klaartje Houben / Jolien J E van Hooff / Agnel-Praveen Joseph / Guido Scarabelli / Barry J Grant / Anthony J Roberts / Maya Topf / Michel O Steinmetz / Marc Baldus / Carolyn A Moores / Anna Akhmanova / ![]() ![]() ![]() ![]() |
PubMed 要旨 | CAMSAP and Patronin family members regulate microtubule minus-end stability and localization and thus organize noncentrosomal microtubule networks, which are essential for cell division, polarization ...CAMSAP and Patronin family members regulate microtubule minus-end stability and localization and thus organize noncentrosomal microtubule networks, which are essential for cell division, polarization and differentiation. Here, we found that the CAMSAP C-terminal CKK domain is widely present among eukaryotes and autonomously recognizes microtubule minus ends. Through a combination of structural approaches, we uncovered how mammalian CKK binds between two tubulin dimers at the interprotofilament interface on the outer microtubule surface. In vitro reconstitution assays combined with high-resolution fluorescence microscopy and cryo-electron tomography suggested that CKK preferentially associates with the transition zone between curved protofilaments and the regular microtubule lattice. We propose that minus-end-specific features of the interprotofilament interface at this site serve as the basis for CKK's minus-end preference. The steric clash between microtubule-bound CKK and kinesin motors explains how CKK protects microtubule minus ends against kinesin-13-induced depolymerization and thus controls the stability of free microtubule minus ends. |
![]() | ![]() ![]() ![]() |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.4 - 8.0 Å |
構造データ | EMDB-3444, PDB-5m5c: EMDB-4154, PDB-5m50: EMDB-4156, PDB-5m54: ![]() PDB-5lzn: |
化合物 | ![]() ChemComp-HOH: ![]() ChemComp-GTP: ![]() ChemComp-MG: ![]() ChemComp-GDP: ![]() ChemComp-TA1: |
由来 |
|
![]() | STRUCTURAL PROTEIN / -TIP / microtubule / CAMSAP CKK Microtubule Tubulin / MOTOR PROTEIN / CAMSAP / CKK / Tubulin / TRANSPORT PROTEIN |