EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 24, Issue 10, Page 809-815, Year 2017
掲載日	2017年9月11日
著者	Carrie Bernecky / Jürgen M Plitzko / Patrick Cramer /
PubMed 要旨	During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA ...During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation complex at a nominal resolution of 3.4 Å. Human DSIF has a modular structure with two domains forming a DNA clamp, two domains forming an RNA clamp, and one domain buttressing the RNA clamp. The clamps maintain the transcription bubble, position upstream DNA, and retain the RNA transcript in the exit tunnel. The mobile C-terminal region of DSIF is located near exiting RNA, where it can recruit factors for RNA processing. The structure provides insight into the roles of DSIF during mRNA synthesis.
リンク	Nat Struct Mol Biol / PubMed:28892040
手法	EM (単粒子) / X線回折
解像度	1.098 - 3.8 Å
構造データ	EMDB-3815: Structure of an RNA polymerase II-DSIF transcription elongation complex 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-3816: Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC2 手法: EM (単粒子) / 解像度: 3.6 Å 3817 5oik EMDB-3817: Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC3 PDB-5oik: Structure of an RNA polymerase II-DSIF transcription elongation complex 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-3818: Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC4 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-3819: Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC5 手法: EM (単粒子) / 解像度: 3.8 Å PDB-5oho: Crystal structure of the KOWx-KOW4 domain of human DSIF 手法: X-RAY DIFFRACTION / 解像度: 1.601 Å PDB-5ohq: Crystal structure of the KOW6-KOW7 domain of human DSIF 手法: X-RAY DIFFRACTION / 解像度: 1.098 Å
化合物	ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-CL: Unknown entry / クロリド ChemComp-HOH: WATER ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	bos taurus (ウシ) homo sapiens (ヒト) synthetic construct (人工物)
キーワード	TRANSCRIPTION / RNA polymerase II / transcription elongation