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Structure paper

TitleStructure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp.
Journal, issue, pagesNat Struct Mol Biol, Vol. 24, Issue 10, Page 809-815, Year 2017
Publish dateSep 11, 2017
AuthorsCarrie Bernecky / Jürgen M Plitzko / Patrick Cramer /
PubMed AbstractDuring transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA ...During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation complex at a nominal resolution of 3.4 Å. Human DSIF has a modular structure with two domains forming a DNA clamp, two domains forming an RNA clamp, and one domain buttressing the RNA clamp. The clamps maintain the transcription bubble, position upstream DNA, and retain the RNA transcript in the exit tunnel. The mobile C-terminal region of DSIF is located near exiting RNA, where it can recruit factors for RNA processing. The structure provides insight into the roles of DSIF during mRNA synthesis.
External linksNat Struct Mol Biol / PubMed:28892040
MethodsEM (single particle) / X-ray diffraction
Resolution1.098 - 3.8 Å
Structure data

EMDB-3815:
Structure of an RNA polymerase II-DSIF transcription elongation complex
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-3816:
Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC2
Method: EM (single particle) / Resolution: 3.6 Å

3817

5oik

EMDB-3817: Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC3
PDB-5oik: Structure of an RNA polymerase II-DSIF transcription elongation complex
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-3818:
Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC4
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-3819:
Structure of an RNA polymerase II-DSIF transcription elongation complex, DSIF-EC5
Method: EM (single particle) / Resolution: 3.8 Å

PDB-5oho:
Crystal structure of the KOWx-KOW4 domain of human DSIF
Method: X-RAY DIFFRACTION / Resolution: 1.601 Å

PDB-5ohq:
Crystal structure of the KOW6-KOW7 domain of human DSIF
Method: X-RAY DIFFRACTION / Resolution: 1.098 Å

Chemicals

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-CL:
Unknown entry / Chloride

ChemComp-HOH:
WATER / Water

ChemComp-NA:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • bos taurus (cattle)
  • homo sapiens (human)
  • synthetic construct (others)
  • Bovine (cattle)
KeywordsTRANSCRIPTION / RNA polymerase II / transcription elongation

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