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Structure paper

TitleStructural basis of MsbA-mediated lipopolysaccharide transport.
Journal, issue, pagesNature, Vol. 549, Issue 7671, Page 233-237, Year 2017
Publish dateSep 14, 2017
AuthorsWei Mi / Yanyan Li / Sung Hwan Yoon / Robert K Ernst / Thomas Walz / Maofu Liao
External linksPubMed:28869968 / Publisher's page
KeywordsATP-Binding Cassette Transporters / Adenosine Diphosphate / Bacterial Proteins / Biological Transport / Cell Membrane / Cryoelectron Microscopy / Escherichia coli / Hydrophobic and Hydrophilic Interactions / Lipid Bilayers / Lipopolysaccharides / Models, Molecular / MsbA protein, Bacteria / Nanostructures / Periplasm / Protein Binding / Protein Domains / HYDROLASE / ABC transporter / LPS / flippase / nanodisc
MethodsEM (single particle)
Resolution4.2 - 6.9 A
Structure data

EMDB-8465:
3D cryo-EM reconstruction of MsbA-nanodisc with ADP

EMDB-8467:
Cryo-EM structure of MsbA-nanodisc with ADP-vanadate

EMDB-8469:
3D cryo-EM reconstruction of nucleotide-free MsbA in lipid nanodisc

EMDB-8669:
CryoEM map of MsbA in POPG nanodisc

EMDB-8670:
CryoEM map of MsbA with lipid A in POPG nanodisc

EMDB-8671:
CryoEM map of MsbA in POPC nanodisc

PDB-5ttp:
Cryo-EM structure of MsbA-nanodisc with ADP-vanadate

PDB-5tv4:
3D cryo-EM reconstruction of nucleotide-free MsbA in lipid nanodisc

Chemicals

ChemComp-PA1:
2-amino-2-deoxy-alpha-D-glucopyranose

ChemComp-KDO:
3-DEOXY-D-MANNO-OCT-2-ULOSONIC ACID3-Deoxy-D-manno-oct-2-ulosonic acid

ChemComp-GMH:
L-GLYCERO-D-MANNO-HEPTOPYRANOSE

ChemComp-PO4:
PHOSPHATE IONPhosphate

ChemComp-FTT:
3-HYDROXY-TETRADECANOIC ACID

ChemComp-MYR:
MYRISTIC ACIDMyristic acid

ChemComp-DAO:
LAURIC ACIDLauric acid

Source
  • Escherichia coli (E. coli)
  • escherichia coli o157:h7 (bacteria)

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