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Structure paper

TitleA conserved threonine prevents self-intoxication of enoyl-thioester reductases.
Journal, issue, pagesNat. Chem. Biol., Vol. 13, Page 745-749, Year 2017
Publish dateJun 15, 2016 (structure data deposition date)
AuthorsRosenthal, R.G. / Vogeli, B. / Wagner, T. / Shima, S. / Erb, T.J.
External linksNat. Chem. Biol. / PubMed:28504678
MethodsX-ray diffraction
Resolution2.1 - 2.5 Å
Structure data

PDB-5lb9:
Structure of the T175V Etr1p mutant in the monoclinic form P21
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5lbx:
Structure of the T175V Etr1p mutant in the trigonal form P312 in complex with NADP and crotonyl-CoA
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-COO:
CROTONYL COENZYME A

ChemComp-CL:
Unknown entry

Source
  • candida tropicalis (yeast)
KeywordsOXIDOREDUCTASE / Negative catalysis / enzyme / NADP / mitochondria / mutant / crotonyl-coa / reductase

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