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Structure paper

TitleStructural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.
Journal, issue, pagesMol. Cell, Vol. 66, Page 221-233.e4, Year 2017
Publish dateMar 16, 2017 (structure data deposition date)
AuthorsSwarts, D.C. / van der Oost, J. / Jinek, M.
External linksMol. Cell / PubMed:28431230
MethodsX-ray diffraction
Resolution2.501 - 3.342 Å
Structure data

PDB-5nfv:
Crystal structure of catalytically inactive FnCas12 mutant bound to an R-loop structure containing a pre-crRNA mimic and full-length DNA target
Method: X-RAY DIFFRACTION / Resolution: 2.501 Å

PDB-5ng6:
Crystal structure of FnCas12a bound to a crRNA
Method: X-RAY DIFFRACTION / Resolution: 3.342 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-B3P:
2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • francisella tularensis subsp. novicida (strain u112) (bacteria)
  • synthetic construct (others)
KeywordsHYDROLASE / CRISPR / Cas / Cpf1 / Cas12a / nuclease / genome editing / R-loop / crRNA / RuvC

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