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TitleDouble-stranded RNA virus outer shell assembly by bona fide domain-swapping.
Journal, issue, pagesNat Commun, Vol. 8, Page 14814, Year 2017
Publish dateMar 13, 2017
AuthorsZhaoyang Sun / Kamel El Omari / Xiaoyu Sun / Serban L Ilca / Abhay Kotecha / David I Stuart / Minna M Poranen / Juha T Huiskonen /
PubMed AbstractCorrect outer protein shell assembly is a prerequisite for virion infectivity in many multi-shelled dsRNA viruses. In the prototypic dsRNA bacteriophage φ6, the assembly reaction is promoted by ...Correct outer protein shell assembly is a prerequisite for virion infectivity in many multi-shelled dsRNA viruses. In the prototypic dsRNA bacteriophage φ6, the assembly reaction is promoted by calcium ions but its biomechanics remain poorly understood. Here, we describe the near-atomic resolution structure of the φ6 double-shelled particle. The outer T=13 shell protein P8 consists of two alpha-helical domains joined by a linker, which allows the trimer to adopt either a closed or an open conformation. The trimers in an open conformation swap domains with each other. Our observations allow us to propose a mechanistic model for calcium concentration regulated outer shell assembly. Furthermore, the structure provides a prime exemplar of bona fide domain-swapping. This leads us to extend the theory of domain-swapping from the level of monomeric subunits and multimers to closed spherical shells, and to hypothesize a mechanism by which closed protein shells may arise in evolution.
External linksNat Commun / PubMed:28287099 / PubMed Central
MethodsEM (single particle)
Resolution4.0 - 9.1 Å
Structure data

3571

5muu

EMDB-3571, PDB-5muu:
dsRNA bacteriophage phi6 nucleocapsid
Method: EM (single particle) / Resolution: 4.0 Å

3572

5muv

EMDB-3572: Localized reconstruction of bacteriophage phi6 packaging hexamer P4
PDB-5muv: Atomic structure fitted into a localized reconstruction of bacteriophage phi6 packaging hexamer P4
Method: EM (single particle) / Resolution: 9.1 Å

3573

5muw

EMDB-3573: Localized reconstruction of bacteriophage phi6 vertex
PDB-5muw: Atomic structure of P4 packaging enzyme fitted into a localized reconstruction of bacteriophage phi6 vertex
Method: EM (single particle) / Resolution: 9.1 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • pseudomonas phage phi6 (bacteriophage)
KeywordsVIRUS / icosahedral virus capsid shell / HYDROLASE / packaging / ATPase / vertex / hyrdolase

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