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Structure paper

TitleDNA recognition by the SwaI restriction endonuclease involves unusual distortion of an 8 base pair A:T-rich target.
Journal, issue, pagesNucleic Acids Res., Vol. 45, Page 1516-1528, Year 2017
Publish dateSep 28, 2016 (structure data deposition date)
AuthorsShen, B.W. / Heiter, D.F. / Lunnen, K.D. / Wilson, G.G. / Stoddard, B.L.
External linksNucleic Acids Res. / PubMed:28180307
MethodsX-ray diffraction
Resolution1.88 - 2.33 Å
Structure data

PDB-5tgq:
Restriction-modification system Type II R.SwaI, DNA free
Method: X-RAY DIFFRACTION / Resolution: 1.88 Å

PDB-5tgx:
Restriction/modification system-Type II R-SwaI complexed with partially cleaved DNA
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-5th3:
Restriction/modification system-Type II R.SwaI cleaved DNA complex
Method: X-RAY DIFFRACTION / Resolution: 2.33 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-MG:
Unknown entry

Source
  • staphylococcus warneri (bacteria)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / rare cutter / apoSwaI / R-SwaI / uncleaved DNA complex / R/M system / I-SwaI / cleaved DNA complex

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