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Structure paper

TitleRestriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.
Journal, issue, pagesNucleic Acids Res., Vol. 45, Page 3547-3558, Year 2017
Publish dateSep 22, 2015 (structure data deposition date)
AuthorsTamulaitiene, G. / Jovaisaite, V. / Tamulaitis, G. / Songailiene, I. / Manakova, E. / Zaremba, M. / Grazulis, S. / Xu, S.Y. / Siksnys, V.
External linksNucleic Acids Res. / PubMed:28039325
MethodsX-ray diffraction
Resolution1.5 - 2.5 Å
Structure data

PDB-5dwa:
Crystal structure of pre-specific restriction endonuclease AgeI-DNA complex
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5dwb:
Crystal structure of specific restriction endonuclease AgeI-DNA complex
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5dwc:
Crystal structure of restriction endonuclease AgeI
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • thalassobius gelatinovorus (bacteria)
  • synthetic construct (others)
KeywordsHYDROLASE / restriction endonuclease / PD-(D/E)XK nuclease / protein-DNA complex

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