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-Structure paper
タイトル | Structural insights into the assembly and regulation of distinct viral capsid complexes. |
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ジャーナル・号・ページ | Nat Commun, Vol. 7, Page 13014, Year 2016 |
掲載日 | 2016年10月4日 |
著者 | Subir Sarker / María C Terrón / Yogesh Khandokar / David Aragão / Joshua M Hardy / Mazdak Radjainia / Manuel Jiménez-Zaragoza / Pedro J de Pablo / Fasséli Coulibaly / Daniel Luque / Shane R Raidal / Jade K Forwood / |
PubMed 要旨 | The assembly and regulation of viral capsid proteins into highly ordered macromolecular complexes is essential for viral replication. Here, we utilize crystal structures of the capsid protein from ...The assembly and regulation of viral capsid proteins into highly ordered macromolecular complexes is essential for viral replication. Here, we utilize crystal structures of the capsid protein from the smallest and simplest known viruses capable of autonomously replicating in animal cells, circoviruses, to establish structural and mechanistic insights into capsid morphogenesis and regulation. The beak and feather disease virus, like many circoviruses, encode only two genes: a capsid protein and a replication initiation protein. The capsid protein forms distinct macromolecular assemblies during replication and here we elucidate these structures at high resolution, showing that these complexes reverse the exposure of the N-terminal arginine rich domain responsible for DNA binding and nuclear localization. We show that assembly of these complexes is regulated by single-stranded DNA (ssDNA), and provide a structural basis of capsid assembly around single-stranded DNA, highlighting novel binding interfaces distinct from the highly positively charged N-terminal ARM domain. |
リンク | Nat Commun / PubMed:27698405 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2 - 4.5 Å |
構造データ | EMDB-8306: PDB-5j09: PDB-5j36: PDB-5j37: |
化合物 | ChemComp-HOH: ChemComp-PO4: |
由来 |
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キーワード | VIRUS / BFDV Virus Capsid Jelly Roll / VIRUS/DNA / VIRAL PROTEIN / VIRUS-DNA complex |