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TitleCryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer.
Journal, issue, pagesScience, Vol. 351, Issue 6277, Page 1043-1048, Year 2016
Publish dateMar 4, 2016
AuthorsJeong Hyun Lee / Gabriel Ozorowski / Andrew B Ward /
PubMed AbstractThe envelope glycoprotein trimer (Env) on the surface of HIV-1 recognizes CD4(+) T cells and mediates viral entry. During this process, Env undergoes substantial conformational rearrangements, making ...The envelope glycoprotein trimer (Env) on the surface of HIV-1 recognizes CD4(+) T cells and mediates viral entry. During this process, Env undergoes substantial conformational rearrangements, making it difficult to study in its native state. Soluble stabilized trimers have provided valuable insights into the Env structure, but they lack the hydrophobic membrane proximal external region (MPER, an important target of broadly neutralizing antibodies), the transmembrane domain, and the cytoplasmic tail. Here we present (i) a cryogenic electron microscopy (cryo-EM) structure of a clade B virus Env, which lacks only the cytoplasmic tail and is stabilized by the broadly neutralizing antibody PGT151, at a resolution of 4.2 angstroms and (ii) a reconstruction of this form of Env in complex with PGT151 and MPER-targeting antibody 10E8 at a resolution of 8.8 angstroms. These structures provide new insights into the wild-type Env structure.
External linksScience / PubMed:26941313 / PubMed Central
MethodsEM (single particle)
Resolution4.19 - 8.8 Å
Structure data

3308

5fuu

EMDB-3308: HIV-1 cleaved wild type JR-FL EnvdCT trimer in complex with PGT151 at 4.19 A resolution
PDB-5fuu: Ectodomain of cleaved wild type JR-FL EnvdCT trimer in complex with PGT151 Fab
Method: EM (single particle) / Resolution: 4.19 Å

EMDB-3309:
HIV-1 cleaved wild type JR-FL EnvdCT trimer in complex with PGT151 at 4.3A resolution
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-3312:
HIV-1 cleaved wild type JR-FL EnvdCT trimer in complex with PGT151 and 10E8 Fabs at 8.8 A resolution
Method: EM (single particle) / Resolution: 8.8 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • Human Immunodeficiency Virus-1
  • homo sapiens (human)
  • human immunodeficiency virus 1
KeywordsVIRAL PROTEIN / HIV-1 / ENV / PGT151 / BROADLY NEUTRALIZING ANTIBODY

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