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Structure paper

TitleStructure and Engineering of Francisella novicida Cas9
Journal, issue, pagesCell, Vol. 164, Page 950-961, Year 2016
Publish dateFeb 1, 2016 (structure data deposition date)
AuthorsHirano, H. / Gootenberg, J.S. / Horii, T. / Abudayyeh, O.O. / Kimura, M. / Hsu, P.D. / Nakane, T. / Ishitani, R. / Hatada, I. / Zhang, F. ...Hirano, H. / Gootenberg, J.S. / Horii, T. / Abudayyeh, O.O. / Kimura, M. / Hsu, P.D. / Nakane, T. / Ishitani, R. / Hatada, I. / Zhang, F. / Nishimasu, H. / Nureki, O.
External linksCell / PubMed:26875867
MethodsX-ray diffraction
Resolution1.7 Å
Structure data

PDB-5b2o:
Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGG PAM)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5b2p:
Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGA PAM)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5b2q:
Crystal structure of Francisella novicida Cas9 RHA in complex with sgRNA and target DNA (TGG PAM)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

Source
  • francisella tularensis subsp. novicida u112 (bacteria)
  • synthetic construct (others)
  • francisella tularensis subsp. novicida (bacteria)
KeywordsHYDROLASE/RNA/DNA / CRISPR-Cas9 / genome engineering / HYDROLASE-RNA-DNA complex

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