+検索条件
-Structure paper
タイトル | Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. |
---|---|
ジャーナル・号・ページ | Structure, Vol. 24, Issue 2, Page 252-260, Year 2016 |
掲載日 | 2016年2月2日 |
![]() | Susan Fetics / Aurélien Thureau / Valérie Campanacci / Magali Aumont-Nicaise / Irène Dang / Alexis Gautreau / Javier Pérez / Jacqueline Cherfils / ![]() |
PubMed 要旨 | Arpin is a newly discovered regulator of actin polymerization at the cell leading edge, which steers cell migration by exerting a negative control on the Arp2/3 complex. Arpin proteins have an acidic ...Arpin is a newly discovered regulator of actin polymerization at the cell leading edge, which steers cell migration by exerting a negative control on the Arp2/3 complex. Arpin proteins have an acidic tail homologous to the acidic motif of the VCA domain of nucleation-promoting factors (NPFs). This tail is predicted to compete with the VCA of NPFs for binding to the Arp2/3 complex, thereby mitigating activation and/or tethering of the complex to sites of actin branching. Here, we investigated the structure of full-length Arpin using synchrotron small-angle X-ray scattering, and of its acidic tail in complex with an ankyrin repeats domain using X-ray crystallography. The data were combined in a hybrid model in which the acidic tail extends from the globular core as a linear peptide and forms a primary epitope that is readily accessible in unbound Arpin and suffices to tether Arpin to interacting proteins with high affinity. |
![]() | ![]() ![]() |
手法 | SAS (X-ray synchrotron) / X線回折 |
解像度 | 1.859 Å |
構造データ | ![]() SASDBT2: Ankyrin repeat domains from human Tankyrase-2 (489-649) ![]() SASDBU2: Human Arpin (isoform 1) (Human Arpin) ![]() SASDBV2: Zebrafish Arpin (Zebrafish (Danio rerio) Arpin, Arpin) ![]() SASDBW2: Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). ![]() SASDBX2: Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469) ![]() PDB-4z68: |
化合物 | ![]() ChemComp-SO4: ![]() ChemComp-HOH: |
由来 |
|
![]() | PROTEIN BINDING / cell migration / Arpin / Arp 2/3 / actin polymerization |