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Title | Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like protein. |
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Journal, issue, pages | EMBO Rep, Vol. 17, Issue 2, Page 235-248, Year 2016 |
Publish date | Dec 28, 2015 |
Authors | Ning Jia / Nan Liu / Wang Cheng / Yong-Liang Jiang / Hui Sun / Lan-Lan Chen / Junhui Peng / Yonghui Zhang / Yue-He Ding / Zhi-Hui Zhang / Xuejuan Wang / Gang Cai / Junfeng Wang / Meng-Qiu Dong / Zhiyong Zhang / Hui Wu / Hong-Wei Wang / Yuxing Chen / Cong-Zhao Zhou / |
PubMed Abstract | Various aerolysin-like pore-forming proteins have been identified from bacteria to vertebrates. However, the mechanism of receptor recognition and/or pore formation of the eukaryotic members remains ...Various aerolysin-like pore-forming proteins have been identified from bacteria to vertebrates. However, the mechanism of receptor recognition and/or pore formation of the eukaryotic members remains unknown. Here, we present the first crystal and electron microscopy structures of a vertebrate aerolysin-like protein from Danio rerio, termed Dln1, before and after pore formation. Each subunit of Dln1 dimer comprises a β-prism lectin module followed by an aerolysin module. Specific binding of the lectin module toward high-mannose glycans triggers drastic conformational changes of the aerolysin module in a pH-dependent manner, ultimately resulting in the formation of a membrane-bound octameric pore. Structural analyses combined with computational simulations and biochemical assays suggest a pore-forming process with an activation mechanism distinct from the previously characterized bacterial members. Moreover, Dln1 and its homologs are ubiquitously distributed in bony fishes and lamprey, suggesting a novel fish-specific defense molecule. |
External links | EMBO Rep / PubMed:26711430 / PubMed Central |
Methods | EM (electron crystallography) / X-ray diffraction |
Resolution | 1.7 - 20.0 Å |
Structure data | EMDB-3244: PDB-4zno: PDB-4znq: PDB-4znr: PDB-5di0: |
Chemicals | ChemComp-CL: ChemComp-EPE: ChemComp-HOH: ChemComp-PEG: ChemComp-PGE: ChemComp-PG4: ChemComp-EDO: ChemComp-1PE: |
Source |
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Keywords | SUGAR BINDING PROTEIN / Pore-forming protein / Aeolysin-like protein / Vetebrate / High-mannose glycans / Complex |