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TitleNovel Inter-Subunit Contacts in Barley Stripe Mosaic Virus Revealed by Cryo-Electron Microscopy.
Journal, issue, pagesStructure, Vol. 23, Issue 10, Page 1815-1826, Year 2015
Publish dateOct 6, 2015
AuthorsDaniel Kofi Clare / Eugenia V Pechnikova / Eugene V Skurat / Valentin V Makarov / Olga S Sokolova / Andrey G Solovyev / Elena V Orlova /
PubMed AbstractBarley stripe mosaic virus (BSMV, genus Hordeivirus) is a rod-shaped single-stranded RNA virus similar to viruses of the structurally characterized and well-studied genus Tobamovirus. Here we report ...Barley stripe mosaic virus (BSMV, genus Hordeivirus) is a rod-shaped single-stranded RNA virus similar to viruses of the structurally characterized and well-studied genus Tobamovirus. Here we report the first high-resolution structure of BSMV at 4.1 Å obtained by cryo-electron microscopy. We discovered that BSMV forms two types of virion that differ in the number of coat protein (CP) subunits per turn and interactions between the CP subunits. While BSMV and tobacco mosaic virus CP subunits have a similar fold and interact with RNA using conserved residues, the axial contacts between the CP of these two viral groups are considerably different. BSMV CP subunits lack substantial axial contacts and are held together by a previously unobserved lateral contact formed at the virion surface via an interacting loop, which protrudes from the CP hydrophobic core to the adjacent CP subunit. These data provide an insight into diversity in structural organization of helical viruses.
External linksStructure / PubMed:26278173 / PubMed Central
MethodsEM (single particle)
Resolution4.1 - 5.8 Å
Structure data

3073

5a79

EMDB-3073, PDB-5a79:
Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by cryo-EM
Method: EM (single particle) / Resolution: 4.1 Å

3074

5a7a

EMDB-3074, PDB-5a7a:
Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by cryo-EM
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-3077:
Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by cryo-EM
Method: EM (single particle) / Resolution: 5.8 Å

EMDB-3078:
Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by cryo-EM
Method: EM (single particle) / Resolution: 5.1 Å

Source
  • tobacco mosaic virus
  • barley stripe mosaic virus
  • tobacco mosaic virus (strain om)
KeywordsVIRUS / BSMV / HELICAL / CRYO-EM / IMAGE PROCESSING / MSA

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