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TitleThe selective autophagy receptor p62 forms a flexible filamentous helical scaffold.
Journal, issue, pagesCell Rep, Vol. 11, Issue 5, Page 748-758, Year 2015
Publish dateMay 5, 2015
AuthorsRodolfo Ciuffa / Trond Lamark / Abul K Tarafder / Audrey Guesdon / Sofia Rybina / Wim J H Hagen / Terje Johansen / Carsten Sachse /
PubMed AbstractThe scaffold protein p62/SQSTM1 is involved in protein turnover and signaling and is commonly found in dense protein bodies in eukaryotic cells. In autophagy, p62 acts as a selective autophagy ...The scaffold protein p62/SQSTM1 is involved in protein turnover and signaling and is commonly found in dense protein bodies in eukaryotic cells. In autophagy, p62 acts as a selective autophagy receptor that recognizes and shuttles ubiquitinated proteins to the autophagosome for degradation. The structural organization of p62 in cellular bodies and the interplay of these assemblies with ubiquitin and the autophagic marker LC3 remain to be elucidated. Here, we present a cryo-EM structural analysis of p62. Together with structures of assemblies from the PB1 domain, we show that p62 is organized in flexible polymers with the PB1 domain constituting a helical scaffold. Filamentous p62 is capable of binding LC3 and addition of long ubiquitin chains induces disassembly and shortening of filaments. These studies explain how p62 assemblies provide a large molecular scaffold for the nascent autophagosome and reveal how they can bind ubiquitinated cargo.
External linksCell Rep / PubMed:25921531
MethodsEM (helical sym.)
Resolution10.3 - 10.9 Å
Structure data

EMDB-2936, PDB-4uf8:
Electron cryo-microscopy structure of PB1-p62 filaments
Method: EM (helical sym.) / Resolution: 10.9 Å

EMDB-2937, PDB-4uf9:
Electron cryo-microscopy structure of PB1-p62 type T filaments
Method: EM (helical sym.) / Resolution: 10.3 Å

Source
  • homo sapiens (human)
KeywordsSelective autophagy / autophagy receptor / autophagy scaffold / p62/SQSTM1 / single-particle helical reconstruction / Adaptor Proteins, Signal Transducing / Amino Acid Sequence / HeLa Cells / Humans / Microscopy, Electron / Microtubule-Associated Proteins / Molecular Sequence Data / Protein Binding / Protein Structure, Secondary / Protein Structure, Tertiary / Recombinant Proteins / SQSTM1 protein, human / Sequestosome-1 Protein / Static Electricity / Ubiquitin / light chain 3, human / SIGNALING PROTEIN

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