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TitleStructure of a biologically active estrogen receptor-coactivator complex on DNA.
Journal, issue, pagesMol Cell, Vol. 57, Issue 6, Page 1047-1058, Year 2015
Publish dateMar 19, 2015
AuthorsPing Yi / Zhao Wang / Qin Feng / Grigore D Pintilie / Charles E Foulds / Rainer B Lanz / Steven J Ludtke / Michael F Schmid / Wah Chiu / Bert W O'Malley /
PubMed AbstractEstrogen receptor (ER/ESR1) is a transcription factor critical for development, reproduction, metabolism, and cancer. ER function hinges on its ability to recruit primary and secondary coactivators, ...Estrogen receptor (ER/ESR1) is a transcription factor critical for development, reproduction, metabolism, and cancer. ER function hinges on its ability to recruit primary and secondary coactivators, yet structural information on the full-length receptor-coactivator complex to complement preexisting and sometimes controversial biochemical information is lacking. Here, we use cryoelectron microscopy (cryo-EM) to determine the quaternary structure of an active complex of DNA-bound ERα, steroid receptor coactivator 3 (SRC-3/NCOA3), and a secondary coactivator (p300/EP300). Our structural model suggests the following assembly mechanism for the complex: each of the two ligand-bound ERα monomers independently recruits one SRC-3 protein via the transactivation domain of ERα; the two SRC-3s in turn bind to different regions of one p300 protein through multiple contacts. We also present structural evidence for the location of activation function 1 (AF-1) in a full-length nuclear receptor, which supports a role for AF-1 in SRC-3 recruitment.
External linksMol Cell / PubMed:25728767 / PubMed Central
MethodsEM (single particle)
Resolution25.0 - 47.0 Å
Structure data

EMDB-6241:
The Structure of a Biologically Active Estrogen Receptor-Coactivator Complex on DNA
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-6259:
ERE-DNA/ERalpha/SRC-3/p300 complex with antibody
Method: EM (single particle) / Resolution: 47.0 Å

EMDB-6260:
ERE-DNA/ERalpha/SRC-3/p300 complexes bind with AF1 antibody
Method: EM (single particle) / Resolution: 35.0 Å

EMDB-6261:
p300
Method: EM (single particle) / Resolution: 42.0 Å

EMDB-6262:
p300 binding with antibody 1
Method: EM (single particle) / Resolution: 31.0 Å

EMDB-6263:
p300 binding with antibody 2
Method: EM (single particle) / Resolution: 32.0 Å

EMDB-6264:
ERE-DNA/ERalpha/SRC-3/p300 complex
Method: EM (single particle) / Resolution: 25.0 Å

Source
  • Homo sapiens (human)
  • unidentified (others)

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