EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Protein synthesis. Rqc2p and 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains.
ジャーナル・号・ページ	Science, Vol. 347, Issue 6217, Page 75-78, Year 2015
掲載日	2015年1月2日
著者	Peter S Shen / Joseph Park / Yidan Qin / Xueming Li / Krishna Parsawar / Matthew H Larson / James Cox / Yifan Cheng / Alan M Lambowitz / Jonathan S Weissman / Onn Brandman / Adam Frost /
PubMed 要旨	In Eukarya, stalled translation induces 40S dissociation and recruitment of the ribosome quality control complex (RQC) to the 60S subunit, which mediates nascent chain degradation. Here we report ...In Eukarya, stalled translation induces 40S dissociation and recruitment of the ribosome quality control complex (RQC) to the 60S subunit, which mediates nascent chain degradation. Here we report cryo-electron microscopy structures revealing that the RQC components Rqc2p (YPL009C/Tae2) and Ltn1p (YMR247C/Rkr1) bind to the 60S subunit at sites exposed after 40S dissociation, placing the Ltn1p RING (Really Interesting New Gene) domain near the exit channel and Rqc2p over the P-site transfer RNA (tRNA). We further demonstrate that Rqc2p recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits. Our work uncovers an unexpected mechanism of protein synthesis, in which a protein--not an mRNA--determines tRNA recruitment and the tagging of nascent chains with carboxy-terminal Ala and Thr extensions ("CAT tails").
リンク	Science / PubMed:25554787 / PubMed Central
手法	EM (単粒子)
解像度	3.5 - 8.4 Å
構造データ	EMDB-2811: Electron cryo-microscopy of yeast 60S ribosome 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-2812: Electron cryo-microscopy of Rqc2 bound to yeast 60S ribosome 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-6169: Electron cryo-microscopy of Rqc2 bound to yeast 60S ribosome, Rqc2-focused alignment 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-6170: Electron cryo-microscopy of Ltn1, Rqc2 bound to yeast 60S ribosome 手法: EM (単粒子) / 解像度: 8.2 Å EMDB-6171: Electron cryo-microscopy of peptidyl-tRNA bound to yeast 60S ribosome 手法: EM (単粒子) / 解像度: 5.9 Å EMDB-6172: Electron cryo-microscopy of Tif6p bound to peptidyl-tRNA-60S ribosomes 手法: EM (単粒子) / 解像度: 5.4 Å EMDB-6173: Electron cryo-microscopy of peptidyl-(~A/P)tRNA-60S particles, ES27out 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-6174: Electron cryo-microscopy of peptidyl-(~A/P)tRNA-60S particles, ES27in 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-6175: Electron cryo-microscopy of peptidyl-(~E and ~P/E)tRNA-60S particles, L1 closed 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-6176: Electron cryo-microscopy of RQC particles purified from Rqc2-deletion yeast strain 手法: EM (単粒子) / 解像度: 8.4 Å EMDB-6201: Electron cryo-microscopy of peptidyl-tRNA bound to yeast 60S ribosome 手法: EM (単粒子) / 解像度: 4.1 Å
由来	Saccharomyces cerevisiae (パン酵母)