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Structure paper

TitleSignificant expansion of fluorescent protein sensing ability through the genetic incorporation of superior photo-induced electron-transfer quenchers.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 136, Page 13094-13097, Year 2014
Publish dateDec 8, 2013 (structure data deposition date)
AuthorsLiu, X. / Jiang, L. / Li, J. / Wang, L. / Yu, Y. / Zhou, Q. / Lv, X. / Gong, W. / Lu, Y. / Wang, J.
External linksJ. Am. Chem. Soc. / PubMed:25197956
MethodsX-ray diffraction
Resolution1.766 - 2.561 Å
Structure data

PDB-4nx2:
Crystal structure of DCYRS complexed with DCY
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4nxb:
Crystal structure of iLOV-I486(2LT) at pH 7.0
Method: X-RAY DIFFRACTION / Resolution: 2.561 Å

PDB-4nxe:
Crystal structure of iLOV-I486(2LT) at pH 6.5
Method: X-RAY DIFFRACTION / Resolution: 2.103 Å

PDB-4nxf:
Crystal structure of iLOV-I486(2LT) at pH 8.0
Method: X-RAY DIFFRACTION / Resolution: 1.766 Å

PDB-4nxg:
Crystal structure of iLOV-I486z(2LT) at pH 9.0
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

Chemicals

ChemComp-2LT:
3,5-dichloro-L-tyrosine

ChemComp-HOH:
WATER

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

Source
  • methanocaldococcus jannaschii (archaea)
  • arabidopsis thaliana (thale cress)
KeywordsLIGASE / Ligase Activity / Translation / Nucleotide / FLAVOPROTEIN / FLUORESCENT PROTEIN / FMN Binding

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