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Structure paper

TitleStructure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 111, Page 652-657, Year 2014
Publish dateMay 14, 2013 (structure data deposition date)
AuthorsSheng, G. / Zhao, H. / Wang, J. / Rao, Y. / Tian, W. / Swarts, D.C. / van der Oost, J. / Patel, D.J. / Wang, Y.
External linksProc. Natl. Acad. Sci. USA / PubMed:24374628
MethodsX-ray diffraction
Resolution2.189 - 2.89 Å
Structure data

PDB-4kpy:
DNA binding protein and DNA complex structure
Method: X-RAY DIFFRACTION / Resolution: 2.406 Å

PDB-4n41:
Structure of Thermus thermophilus Argonaute bound to guide DNA and 15-mer target DNA
Method: X-RAY DIFFRACTION / Resolution: 2.248 Å

PDB-4n47:
Structure of Thermus thermophilus Argonaute bound to guide DNA and 12-mer target DNA
Method: X-RAY DIFFRACTION / Resolution: 2.823 Å

PDB-4n76:
Structure of Thermus thermophilus Argonaute bound to guide DNA and cleaved target DNA with Mn2+
Method: X-RAY DIFFRACTION / Resolution: 2.89 Å

PDB-4nca:
Structure of Thermus thermophilus Argonaute bound to guide DNA 19-mer and target DNA in the presence of Mg2+
Method: X-RAY DIFFRACTION / Resolution: 2.489 Å

PDB-4ncb:
Structure of Thermus thermophilus Argonaute bound to guide DNA and 19-mer target DNA with Mg2+
Method: X-RAY DIFFRACTION / Resolution: 2.189 Å

Chemicals

ChemComp-TMP:
THYMIDINE-5'-PHOSPHATE

ChemComp-MN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

Source
  • thermus thermophilus (bacteria)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN/DNA / Argonaute / RNA interference / DNA interference / DNA BINDING PROTEIN-DNA complex / NUCLEAR PROTEIN/DNA / NUCLEAR PROTEIN-DNA complex / Piwi

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