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Structure paper

TitleCrystal Structure and Site-Directed Mutagenesis of 3-Ketosteroid Delta1-Dehydrogenase from Rhodococcus Erythropolis Sq1 Explain its Catalytic Mechanism
Journal, issue, pagesJ. Biol. Chem., Vol. 288, Page 35559-, Year 2013
Publish dateAug 28, 2013 (structure data deposition date)
AuthorsRohman, A. / Van Oosterwijk, N. / Thunnissen, A.M.W.H. / Dijkstra, B.W.
External linksJ. Biol. Chem. / PubMed:24165124
MethodsX-ray diffraction
Resolution2 - 2.3 Å
Structure data

PDB-4c3x:
Crystal structure of 3-ketosteroid delta1-dehydrogenase from Rhodococcus erythropolis SQ1
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4c3y:
Crystal structure of 3-ketosteroid delta1-dehydrogenase from Rhodococcus erythropolis SQ1 in complex with 1,4-androstadiene-3,17- dione
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-ANB:
ANDROSTA-1,4-DIENE-3,17-DIONE / hormone*YM

Source
  • rhodococcus erythropolis (bacteria)
KeywordsOXIDOREDUCTASE / FLAVOPROTEIN / ROSSMANN FOLD

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