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Structure paper

TitleStructural insights into the hydrolysis and polymorphism of methotrexate polyglutamate by zebrafish gamma-glutamyl hydrolase
Journal, issue, pagesJ. Med. Chem., Vol. 56, Page 7625-7635, Year 2013
Publish dateJun 14, 2013 (structure data deposition date)
AuthorsChuankhayan, P. / Kao, T.-T. / Lin, C.-C. / Guan, H.-H. / Nakagawa, A. / Fu, T.-F. / Chen, C.-J.
External linksJ. Med. Chem. / PubMed:24028568
MethodsX-ray diffraction
Resolution1.97 - 2.39 Å
Structure data

PDB-4l7q:
Crystal structure of gamma glutamyl hydrolase (wild-type) from zebrafish
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-4l8f:
Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX
Method: X-RAY DIFFRACTION / Resolution: 1.97 Å

PDB-4l8w:
Crystal structure of gamma glutamyl hydrolase (H218N) from zebrafish complex with MTX polyglutamate
Method: X-RAY DIFFRACTION / Resolution: 2.39 Å

PDB-4l8y:
Crystal structure of gamma-glutamyl hydrolase (C108A) from zebrafish
Method: X-RAY DIFFRACTION / Resolution: 1.97 Å

PDB-4l95:
Crystal structure of gamma glutamyl hydrolase (H218N) from zebrafish
Method: X-RAY DIFFRACTION / Resolution: 2.34 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-MTX:
METHOTREXATE / chemotherapy*YM

ChemComp-DGL:
D-GLUTAMIC ACID

Source
  • danio rerio (zebrafish)
KeywordsHYDROLASE / sandwiched-like domains / Gamma-glutamyl Hydrolase / Sandwiched-like domain

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