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TitleA refined model of the prototypical Salmonella SPI-1 T3SS basal body reveals the molecular basis for its assembly.
Journal, issue, pagesPLoS Pathog, Vol. 9, Issue 4, Page e1003307, Year 2013
Publish dateApr 25, 2013
AuthorsJulien R C Bergeron / Liam J Worrall / Nikolaos G Sgourakis / Frank DiMaio / Richard A Pfuetzner / Heather B Felise / Marija Vuckovic / Angel C Yu / Samuel I Miller / David Baker / Natalie C J Strynadka /
PubMed AbstractThe T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed ...The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed of a "basal body", a lock-nut structure spanning both bacterial membranes, and a "needle" that protrudes away from the bacterial surface. A hollow channel spans throughout the apparatus, permitting the translocation of effector proteins from the bacterial cytosol to the host plasma membrane. The basal body is composed largely of three membrane-embedded proteins that form oligomerized concentric rings. Here, we report the crystal structures of three domains of the prototypical Salmonella SPI-1 basal body, and use a new approach incorporating symmetric flexible backbone docking and EM data to produce a model for their oligomeric assembly. The obtained models, validated by biochemical and in vivo assays, reveal the molecular details of the interactions driving basal body assembly, and notably demonstrate a conserved oligomerization mechanism.
External linksPLoS Pathog / PubMed:23633951 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.801 - 11.7 Å
Structure data

PDB-3j1v:
A refined model of the prototypical Salmonella typhimurium T3SS basal body reveals the molecular basis for its assembly
Method: ELECTRON MICROSCOPY / Resolution: 11.7 Å

PDB-3j1w:
A refined model of the prototypical Salmonella typhimurium T3SS basal body reveals the molecular basis for its assembly
Method: ELECTRON MICROSCOPY / Resolution: 11.7 Å

PDB-3j1x:
A refined model of the prototypical Salmonella typhimurium T3SS basal body reveals the molecular basis for its assembly
Method: ELECTRON MICROSCOPY / Resolution: 11.7 Å

PDB-4g08:
Crystal structure of the periplasmic domain of InvG
Method: X-RAY DIFFRACTION / Resolution: 1.801 Å

PDB-4g1i:
Structure of the PrgH periplasmic domain
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-4g2s:
Crystal structure of a Salmonella type III secretion system protein
Method: X-RAY DIFFRACTION / Resolution: 1.858 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-PO4:
PHOSPHATE ION

ChemComp-CA:
Unknown entry

Source
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
KeywordsCELL INVASION / T3SS / Secretin / Ring-building motif / protein secretion / PrgH / Type III secretion / InvG / FHA domain

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