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Structure paper

TitleThe structural basis of force generation by the mitotic motor kinesin-5.
Journal, issue, pagesJ Biol Chem, Vol. 287, Issue 53, Page 44654-44666, Year 2012
Publish dateDec 28, 2012
AuthorsAdeline Goulet / William M Behnke-Parks / Charles V Sindelar / Jennifer Major / Steven S Rosenfeld / Carolyn A Moores /
PubMed AbstractKinesin-5 is required for forming the bipolar spindle during mitosis. Its motor domain, which contains nucleotide and microtubule binding sites and mechanical elements to generate force, has evolved ...Kinesin-5 is required for forming the bipolar spindle during mitosis. Its motor domain, which contains nucleotide and microtubule binding sites and mechanical elements to generate force, has evolved distinct properties for its spindle-based functions. In this study, we report subnanometer resolution cryoelectron microscopy reconstructions of microtubule-bound human kinesin-5 before and after nucleotide binding and combine this information with studies of the kinetics of nucleotide-induced neck linker and cover strand movement. These studies reveal coupled, nucleotide-dependent conformational changes that explain many of this motor's properties. We find that ATP binding induces a ratchet-like docking of the neck linker and simultaneous, parallel docking of the N-terminal cover strand. Loop L5, the binding site for allosteric inhibitors of kinesin-5, also undergoes a dramatic reorientation when ATP binds, suggesting that it is directly involved in controlling nucleotide binding. Our structures indicate that allosteric inhibitors of human kinesin-5, which are being developed as anti-cancer therapeutics, bind to a motor conformation that occurs in the course of normal function. However, due to evolutionarily defined sequence variations in L5, this conformation is not adopted by invertebrate kinesin-5s, explaining their resistance to drug inhibition. Together, our data reveal the precision with which the molecular mechanism of kinesin-5 motors has evolved for force generation.
External linksJ Biol Chem / PubMed:23135273 / PubMed Central
MethodsEM (single particle)
Resolution9.5 - 15.0 Å
Structure data

2077

4aqv

EMDB-2077: Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in AMPPNP state.
PDB-4aqv: Model of human kinesin-5 motor domain (3HQD) and mammalian tubulin heterodimer (1JFF) docked into the 9.7-angstrom cryo-EM map of microtubule-bound kinesin-5 motor domain in the AMPPPNP state.
Method: EM (single particle) / Resolution: 9.7 Å

2078

4aqw

EMDB-2078: Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in rigor state
PDB-4aqw: Model of human kinesin-5 motor domain (1II6, 3HQD) and mammalian tubulin heterodimer (1JFF) docked into the 9.5-angstrom cryo-EM map of microtubule-bound kinesin-5 motor domain in the rigor state.
Method: EM (single particle) / Resolution: 9.5 Å

EMDB-2079:
Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in rigor state (gold cluster in loop5 T126C).
Method: EM (single particle) / Resolution: 11.0 Å

EMDB-2080:
Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in rigor state (gold cluster in the neck linker V365C).
Method: EM (single particle) / Resolution: 11.0 Å

EMDB-2081:
Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in the AMPPNP state (gold cluster in the neck linker V365C).
Method: EM (single particle) / Resolution: 15.0 Å

EMDB-2152:
Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in the AMPPNP state (gold cluster in the loop5 T126C).
Method: EM (single particle) / Resolution: 12.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • bos taurus (cattle)
  • homo sapiens (human)
KeywordsMOTOR PROTEIN / MICROTUBULE / MITOSIS / CANCER

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