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TitleThe Last Piece in the Vitamin B1 Biosynthesis Puzzle: STRUCTURAL AND FUNCTIONAL INSIGHT INTO YEAST 4-AMINO-5-HYDROXYMETHYL-2-METHYLPYRIMIDINE PHOSPHATE (HMP-P) SYNTHASE.
Journal, issue, pagesJ. Biol. Chem., Vol. 287, Page 42333-42343, Year 2012
Publish dateSep 19, 2012 (structure data deposition date)
AuthorsCoquille, S. / Roux, C. / Fitzpatrick, T.B. / Thore, S.
External linksJ. Biol. Chem. / PubMed:23048037
MethodsX-ray diffraction
Resolution2.6 - 2.9 Å
Structure data

PDB-4h65:
Crystal structure of SeMet derivative of HMP synthase Thi5 from S. cerevisiae
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-4h67:
Crystal structure of HMP synthase Thi5 from S. cerevisiae
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-4h6d:
Crystal structure of PLP-soaked HMP synthase Thi5 from S. cerevisiae
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSFERASE / HMP-P synthase / THI5-PLP complex / PLP binding / Synthase

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