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TitleFabs enable single particle cryoEM studies of small proteins.
Journal, issue, pagesStructure, Vol. 20, Issue 4, Page 582-592, Year 2012
Publish dateApr 4, 2012
AuthorsShenping Wu / Agustin Avila-Sakar / JungMin Kim / David S Booth / Charles H Greenberg / Andrea Rossi / Maofu Liao / Xueming Li / Akram Alian / Sarah L Griner / Narinobu Juge / Yadong Yu / Claudia M Mergel / Javier Chaparro-Riggers / Pavel Strop / Robert Tampé / Robert H Edwards / Robert M Stroud / Charles S Craik / Yifan Cheng /
PubMed AbstractIn spite of its recent achievements, the technique of single particle electron cryomicroscopy (cryoEM) has not been widely used to study proteins smaller than 100 kDa, although it is a highly ...In spite of its recent achievements, the technique of single particle electron cryomicroscopy (cryoEM) has not been widely used to study proteins smaller than 100 kDa, although it is a highly desirable application of this technique. One fundamental limitation is that images of small proteins embedded in vitreous ice do not contain adequate features for accurate image alignment. We describe a general strategy to overcome this limitation by selecting a fragment antigen binding (Fab) to form a stable and rigid complex with a target protein, thus providing a defined feature for accurate image alignment. Using this approach, we determined a three-dimensional structure of an ∼65 kDa protein by single particle cryoEM. Because Fabs can be readily generated against a wide range of proteins by phage display, this approach is generally applicable to study many small proteins by single particle cryoEM.
External linksStructure / PubMed:22483106 / PubMed Central
MethodsEM (single particle)
Resolution9.6 - 25.0 Å
Structure data

EMDB-5294:
3D reconstruction of frozen hydrated HIV-1 integrase dimer in complex with two Fabs.
Method: EM (single particle) / Resolution: 9.6 Å

EMDB-5295:
3D reconstruction of negatively stained PCSK9 in complex with a Fab
Method: EM (single particle) / Resolution: 25.0 Å

Source
  • Human immunodeficiency virus 1
  • Homo sapiens (human)

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