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TitleMolecular rearrangements involved in the capsid shell maturation of bacteriophage T7.
Journal, issue, pagesJ Biol Chem, Vol. 286, Issue 1, Page 234-242, Year 2011
Publish dateJan 7, 2011
AuthorsAlina Ionel / Javier A Velázquez-Muriel / Daniel Luque / Ana Cuervo / José R Castón / José M Valpuesta / Jaime Martín-Benito / José L Carrascosa /
PubMed AbstractMaturation of dsDNA bacteriophages involves assembling the virus prohead from a limited set of structural components followed by rearrangements required for the stability that is necessary for ...Maturation of dsDNA bacteriophages involves assembling the virus prohead from a limited set of structural components followed by rearrangements required for the stability that is necessary for infecting a host under challenging environmental conditions. Here, we determine the mature capsid structure of T7 at 1 nm resolution by cryo-electron microscopy and compare it with the prohead to reveal the molecular basis of T7 shell maturation. The mature capsid presents an expanded and thinner shell, with a drastic rearrangement of the major protein monomers that increases in their interacting surfaces, in turn resulting in a new bonding lattice. The rearrangements include tilting, in-plane rotation, and radial expansion of the subunits, as well as a relative bending of the A- and P-domains of each subunit. The unique features of this shell transformation, which does not employ the accessory proteins, inserted domains, or molecular interactions observed in other phages, suggest a simple capsid assembling strategy that may have appeared early in the evolution of these viruses.
External linksJ Biol Chem / PubMed:20962334 / PubMed Central
MethodsEM (single particle)
Resolution10.8 - 10.9 Å
Structure data

1810

2xvr

EMDB-1810: Phage T7 empty head.
PDB-2xvr: Phage T7 empty mature head shell
Method: EM (single particle) / Resolution: 10.8 Å

PDB-3izg:
Bacteriophage T7 prohead shell EM-derived atomic model
Method: ELECTRON MICROSCOPY / Resolution: 10.9 Å

Source
  • enterobacteria phage t7 (virus)
KeywordsVIRUS / CAPSID MATURATION / MORPHOGENETIC INTERMEDIATE / bacteriophage / cryoelectron microscopy / icosahedral

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