タイトル | Structural evidence for enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D3 hydroxylase |
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ジャーナル・号・ページ | J. Biol. Chem., Vol. 285, Page 31193-31201, Year 2010 |
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掲載日 | 2009年7月7日 (構造データの登録日) |
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著者 | Yasutake, Y. / Fujii, Y. / Nishioka, T. / Cheon, W.K. / Arisawa, A. / Tamura, T. |
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リンク | J. Biol. Chem. / PubMed:20667833 |
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手法 | X線回折 |
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解像度 | 1.75 - 3.06 Å |
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構造データ | PDB-3a4g: Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form) 手法: X-RAY DIFFRACTION / 解像度: 1.75 Å PDB-3a4h: Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (orthorhombic crystal form) 手法: X-RAY DIFFRACTION / 解像度: 3.06 Å PDB-3a4z: Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å PDB-3a50: Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution with bound vitamin D3 手法: X-RAY DIFFRACTION / 解像度: 2.05 Å PDB-3a51: Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution with bound 25-hydroxyvitamin D3 手法: X-RAY DIFFRACTION / 解像度: 2 Å |
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化合物 | ChemComp-VD3: (1S,3Z)-3-[(2E)-2-[(1R,3AR,7AS)-7A-METHYL-1-[(2R)-6-METHYLHEPTAN-2-YL]-2,3,3A,5,6,7-HEXAHYDRO-1H-INDEN-4-YLIDENE]ETHYLI
ChemComp-VDY: 3-{2-[1-(5-HYDROXY-1,5-DIMETHYL-HEXYL)-7A-METHYL-OCTAHYDRO-INDEN-4-YLIDENE]-ETHYLIDENE}-4-METHYLENE-CYCLOHEXANOL / 25-ヒドロキシビタミンD3
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由来 | - pseudonocardia autotrophica (バクテリア)
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キーワード | OXIDOREDUCTASE / Cytochrome P450 / vitamin D3 hydroxylase / hemoprotein / Monooxygenase / directed evolution |
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