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TitleSwitching catalysis from hydrolysis to perhydrolysis in Pseudomonas fluorescens esterase.
Journal, issue, pagesBiochemistry, Vol. 49, Page 1931-1942, Year 2010
Publish dateMay 8, 2009 (structure data deposition date)
AuthorsYin de, L.T. / Bernhardt, P. / Morley, K.L. / Jiang, Y. / Cheeseman, J.D. / Purpero, V. / Schrag, J.D. / Kazlauskas, R.J.
External linksBiochemistry / PubMed:20112920
MethodsX-ray diffraction
Resolution1.9 - 2.25 Å
Structure data

PDB-3hea:
The L29P/L124I mutation of Pseudomonas fluorescens esterase
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3hi4:
Switching catalysis from hydrolysis to perhydrolysis in P. fluorescens esterase
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-EEE:
ETHYL ACETATE

ChemComp-HOH:
WATER

ChemComp-ACT:
ACETATE ION

Source
  • pseudomonas fluorescens (bacteria)
KeywordsHYDROLASE / alpha/beta hydrolase / esterase / covalent adduct / tetrahedral intermediate / Oxidoreductase / Peroxidase / hydrolysis / perhydrolysis

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