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-Structure paper
タイトル | Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus. |
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ジャーナル・号・ページ | Science, Vol. 326, Issue 5957, Page 1279-1283, Year 2009 |
掲載日 | 2009年11月27日 |
著者 | Rajiv G Tawar / Stéphane Duquerroy / Clemens Vonrhein / Paloma F Varela / Laurence Damier-Piolle / Nathalie Castagné / Kirsty MacLellan / Hugues Bedouelle / Gérard Bricogne / David Bhella / Jean-François Eléouët / Félix A Rey / |
PubMed 要旨 | The respiratory syncytial virus (RSV) is an important human pathogen, yet neither a vaccine nor effective therapies are available to treat infection. To help elucidate the replication mechanism of ...The respiratory syncytial virus (RSV) is an important human pathogen, yet neither a vaccine nor effective therapies are available to treat infection. To help elucidate the replication mechanism of this RNA virus, we determined the three-dimensional (3D) crystal structure at 3.3 A resolution of a decameric, annular ribonucleoprotein complex of the RSV nucleoprotein (N) bound to RNA. This complex mimics one turn of the viral helical nucleocapsid complex, which serves as template for viral RNA synthesis. The RNA wraps around the protein ring, with seven nucleotides contacting each N subunit, alternating rows of four and three stacked bases that are exposed and buried within a protein groove, respectively. Combined with electron microscopy data, this structure provides a detailed model for the RSV nucleocapsid, in which the bases are accessible for readout by the viral polymerase. Furthermore, the nucleoprotein structure highlights possible key sites for drug targeting. |
リンク | Science / PubMed:19965480 |
手法 | EM (らせん対称) / X線回折 |
解像度 | 3.29 - 26.0 Å |
構造データ | EMDB-1622: PDB-2wj8: |
化合物 | ChemComp-BO4: ChemComp-HOH: |
由来 |
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キーワード | RNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN RNA COMPLEX / NUCLEOCAPSID (N) / RIBONUCLEOPROTEIN / RESPIRATORY SYNCYTIAL VIRUS (RSV) / VIRAL NUCLEOPROTEIN / TEMPLATE-LIKE ASSEMBLY / RNA / VIRION / COMPLEX / CYTOPLASM / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex |