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TitleCrystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus.
Journal, issue, pagesScience, Vol. 326, Issue 5957, Page 1279-1283, Year 2009
Publish dateNov 27, 2009
AuthorsRajiv G Tawar / Stéphane Duquerroy / Clemens Vonrhein / Paloma F Varela / Laurence Damier-Piolle / Nathalie Castagné / Kirsty MacLellan / Hugues Bedouelle / Gérard Bricogne / David Bhella / Jean-François Eléouët / Félix A Rey /
PubMed AbstractThe respiratory syncytial virus (RSV) is an important human pathogen, yet neither a vaccine nor effective therapies are available to treat infection. To help elucidate the replication mechanism of ...The respiratory syncytial virus (RSV) is an important human pathogen, yet neither a vaccine nor effective therapies are available to treat infection. To help elucidate the replication mechanism of this RNA virus, we determined the three-dimensional (3D) crystal structure at 3.3 A resolution of a decameric, annular ribonucleoprotein complex of the RSV nucleoprotein (N) bound to RNA. This complex mimics one turn of the viral helical nucleocapsid complex, which serves as template for viral RNA synthesis. The RNA wraps around the protein ring, with seven nucleotides contacting each N subunit, alternating rows of four and three stacked bases that are exposed and buried within a protein groove, respectively. Combined with electron microscopy data, this structure provides a detailed model for the RSV nucleocapsid, in which the bases are accessible for readout by the viral polymerase. Furthermore, the nucleoprotein structure highlights possible key sites for drug targeting.
External linksScience / PubMed:19965480
MethodsEM (helical sym.) / X-ray diffraction
Resolution3.29 - 26.0 Å
Structure data

EMDB-1622:
Helical reconstruction of Respiratory Syncytial Virus N-RNA helices
Method: EM (helical sym.) / Resolution: 26.0 Å

PDB-2wj8:
Respiratory Syncitial Virus RiboNucleoProtein
Method: X-RAY DIFFRACTION / Resolution: 3.29 Å

Chemicals

ChemComp-BO4:
BORATE ION

ChemComp-HOH:
WATER

Source
  • Respiratory syncytial virus
  • human respiratory syncytial virus a strain long
  • escherichia coli (E. coli)
KeywordsRNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN RNA COMPLEX / NUCLEOCAPSID (N) / RIBONUCLEOPROTEIN / RESPIRATORY SYNCYTIAL VIRUS (RSV) / VIRAL NUCLEOPROTEIN / TEMPLATE-LIKE ASSEMBLY / RNA / VIRION / COMPLEX / CYTOPLASM / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex

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