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Structure paper

TitleStructure of RNase Sa2 complexes with mononucleotides - new aspects of catalytic reaction and substrate recognition
Journal, issue, pagesFebs J., Vol. 276, Page 4156-4168, Year 2009
Publish dateMay 14, 2008 (structure data deposition date)
AuthorsBauerova-Hlinkova, V. / Dvorsky, R. / Perecko, D. / Povazanec, F. / Sevcik, J.
External linksFebs J. / PubMed:19558492
MethodsX-ray diffraction
Resolution1.8 - 2.25 Å
Structure data

PDB-3d4a:
Crystal structure of ribonuclease Sa2 with 3'-GMP obtained by ligand diffusion
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-3d5g:
Structure of ribonuclease Sa2 complexes with mononucleotides: new aspects of catalytic reaction and substrate recognition
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3d5i:
Crystal structure of ribonuclease Sa2 with exo-2',3'-cyclophosphorotioate
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-3dgy:
Crystal structure of ribonuclease Sa2 with guanosine-2'-cyclophosphate
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3dh2:
Crystal structure of ribonuclease Sa2 with guanosine-3'-cyclophosphate prepared by cocrystallization
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-3GP:
GUANOSINE-3'-MONOPHOSPHATE

ChemComp-HOH:
WATER

ChemComp-SGP:
GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-2GP:
GUANOSINE-2'-MONOPHOSPHATE

Source
  • streptomyces aureofaciens (bacteria)
KeywordsHYDROLASE / ribonuclease / mononucleotide / protein-mononucleotide complex / exo-2' / 3'-cyclophosphorotioate / guanosine-2'-monophosphate / guanosine-3'-monophosphate / cocrystallization

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