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TitleStructure of the kinesin13-microtubule ring complex.
Journal, issue, pagesStructure, Vol. 16, Issue 11, Page 1732-1739, Year 2008
Publish dateNov 12, 2008
AuthorsDongyan Tan / William J Rice / Hernando Sosa /
PubMed AbstractTo investigate the mechanism of kinesin13-induced microtubule depolymerization, we have calculated a three-dimensional (3D) map of the kinesin13-microtubule ring complex, using cryo-electron ...To investigate the mechanism of kinesin13-induced microtubule depolymerization, we have calculated a three-dimensional (3D) map of the kinesin13-microtubule ring complex, using cryo-electron microscopy (cryo-EM) and image analysis. An atomic model of the complex was produced by docking the crystal structures of tubulin and a kinesin13 motor domain (MD) into the 3D map. The model reveals a snapshot of the depolymerization mechanism by providing a 3D view of the complex formed between the kinesin13 MD and a curved tubulin protofilament (pf). It suggests that contacts mediated by kinesin13 class-specific residues in the putative microtubule-binding site stabilize intra-dimer tubulin curvature. In addition, a tubulin-binding site on the kinesin13 MD was identified. Mutations at this class-conserved site selectively disrupt the formation of microtubule-associated ring complexes.
External linksStructure / PubMed:19000825 / PubMed Central
MethodsEM (helical sym.)
Resolution28.0 Å
Structure data

5027

3edl

EMDB-5027: Kinesin13-Microtubule Ring Complex
PDB-3edl: Kinesin13-Microtubule Ring complex
Method: EM (helical sym.) / Resolution: 28.0 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-CN2:
2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE

ChemComp-HOH:
WATER

Source
  • drosophila melanogaster (fruit fly)
  • bos taurus (domestic cattle)
KeywordsSTRUCTURAL PROTEIN / Kinesin / Kinesin13 / Kin-I / M-Kinesin / Microtubule / Tubulin / depolymerization

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