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Structure paper

TitleStructural analysis of the heterodimeric type IIS restriction endonuclease R.BspD6I acting as a complex between a monomeric site-specific nickase and a catalytic subunit.
Journal, issue, pagesJ. Mol. Biol., Vol. 384, Page 489-502, Year 2008
Publish dateNov 3, 2005 (structure data deposition date)
AuthorsKachalova, G.S. / Rogulin, E.A. / Yunusova, A.K. / Artyukh, R.I. / Perevyazova, T.A. / Matvienko, N.I. / Zheleznaya, L.A. / Bartunik, H.D.
External linksJ. Mol. Biol. / PubMed:18835275
MethodsX-ray diffraction
Resolution1.5 - 1.84 Å
Structure data

PDB-2ewf:
Crystal structure of the site-specific DNA nickase N.BspD6I
Method: X-RAY DIFFRACTION / Resolution: 1.84 Å

PDB-2p14:
Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-BR:
BROMIDE ION

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

Source
  • bacillus sp. (bacteria)
KeywordsHYDROLASE / helix-turn-helix / beta-alpha-barrel / heterodimeric restriction endonuclease

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