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Structure paper

TitleThe Case of the Missing Ring: Radical Cleavage of a Carbon-Carbon Bond and Implications for GFP Chromophore Biosynthesis
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 129, Page 3118-3126, Year 2007
Publish dateJun 16, 2006 (structure data deposition date)
AuthorsBarondeau, D.P. / Kassmann, C.J. / Tainer, J.A. / Getzoff, E.D.
External linksJ. Am. Chem. Soc. / PubMed:17326633
MethodsX-ray diffraction
Resolution1.2 - 2.05 Å
Structure data

PDB-2hcg:
Structure of S65T Y66F GFP variant after cyclization, carbon-carbon bond cleavage, and oxygen incorporation reactions
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-2hfc:
Structure of S65T Y66F R96A GFP variant in precursor state
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-2hgd:
Structure of S65A Y66F GFP variant with an oxidized chromophore
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-2hgy:
Structure of S65A Y66F E222A GFP variant after cyclization and carbon-carbon bond cleavage
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • aequorea victoria (jellyfish)
KeywordsLUMINESCENT PROTEIN / post-translational modification / cyclization / cleavage / fluorophore / X-ray damage / oxidation / GFP / carbon-carbon bond cleavage / radical

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