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Structure paper

TitleTime-Resolved Fluorescence of 2-Aminopurine as a Probe of Base Flipping in M.HhaI-DNA Complexes.
Journal, issue, pagesNucleic Acids Res., Vol. 33, Page 6953-, Year 2005
Publish dateNov 25, 2005 (structure data deposition date)
AuthorsNeely, R.K. / Daujotyte, D. / Grazulis, S. / Magennis, S.W. / Dryden, D.T.F. / Klimasauskas, S. / Jones, A.C.
External linksNucleic Acids Res. / PubMed:16340006
MethodsX-ray diffraction
Resolution1.7 - 1.9 Å
Structure data

PDB-2c7o:
HhaI DNA methyltransferase complex with 13mer oligonucleotide containing 2-aminopurine adjacent to the target base (PCGC:GMGC) and SAH
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-2c7p:
HhaI DNA methyltransferase complex with oligonucleotide containing 2- aminopurine opposite to the target base (GCGC:GMPC) and SAH
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-2c7q:
HhaI DNA methyltransferase complex with oligonucleotide containing 2- aminopurine outside the recognition sequence (paired with G) and SAH
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-2c7r:
HhaI DNA methyltransferase (T250G mutant) complex with oligonucleotide containing 2-aminopurine as a target base (GPGC:GMGC) and SAH
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-CIT:
CITRIC ACID

ChemComp-GOL:
GLYCEROL

Source
  • haemophilus haemolyticus (bacteria)
  • synthetic construct (others)
KeywordsTRANSFERASE/DNA / TRANSFERASE-DNA COMPLEX / BASE FLIPPING / TRANSFERASE RESTRICTION SYSTEM / RESTRICTION SYSTEM / TRANSFERASE

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