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TitleStructural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 5003, Year 2020
Publish dateOct 6, 2020
AuthorsJinfan Wang / Jing Wang / Byung-Sik Shin / Joo-Ran Kim / Thomas E Dever / Joseph D Puglisi / Israel S Fernández /
PubMed AbstractRecognition of a start codon by the initiator aminoacyl-tRNA determines the reading frame of messenger RNA (mRNA) translation by the ribosome. In eukaryotes, the GTPase eIF5B collaborates in the ...Recognition of a start codon by the initiator aminoacyl-tRNA determines the reading frame of messenger RNA (mRNA) translation by the ribosome. In eukaryotes, the GTPase eIF5B collaborates in the correct positioning of the initiator Met-tRNA on the ribosome in the later stages of translation initiation, gating entrance into elongation. Leveraging the long residence time of eIF5B on the ribosome recently identified by single-molecule fluorescence measurements, we determine the cryoEM structure of the naturally long-lived ribosome complex with eIF5B and Met-tRNA immediately before transition into elongation. The structure uncovers an unexpected, eukaryotic specific and dynamic fidelity checkpoint implemented by eIF5B in concert with components of the large ribosomal subunit.
External linksNat Commun / PubMed:33024099
MethodsEM (single particle)
Resolution2.9 Å
Structure data

21859

6woo

EMDB-21859, PDB-6woo:
CryoEM structure of yeast 80S ribosome with Met-tRNAiMet, eIF5B, and GDP
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-ZN:
ZINC ION / Zinc

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-U6A:
N-carboxy-L-threonine

ChemComp-MET:
METHIONINE / Methionine

Source
  • saccharomyces cerevisiae (baker's yeast)
  • Baker's yeast (baker's yeast)
KeywordsRIBOSOME / yeast / initiation / eIF5B / GDP / Met-tRNAiMet

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