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TitleCore architecture of a bacterial type II secretion system.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 5437, Year 2019
Publish dateNov 28, 2019
AuthorsAnastasia A Chernyatina / Harry H Low /
PubMed AbstractBacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here we use negative stain and cryo-electron microscopy to reveal the ...Bacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here we use negative stain and cryo-electron microscopy to reveal the core architecture of an assembled T2SS from the pathogen Klebsiella pneumoniae. We show that 7 proteins form a ~2.4 MDa complex that spans the cell envelope. The outer membrane complex includes the secretin PulD, with all domains modelled, and the pilotin PulS. The inner membrane assembly platform components PulC, PulE, PulL, PulM and PulN have a relative stoichiometric ratio of 2:1:1:1:1. The PulE ATPase, PulL and PulM combine to form a flexible hexameric hub. Symmetry mismatch between the outer membrane complex and assembly platform is overcome by PulC linkers spanning the periplasm, with PulC HR domains binding independently at the secretin base. Our results show that the T2SS has a highly dynamic modular architecture, with implication for pseudo-pilus assembly and substrate loading.
External linksNat Commun / PubMed:31780649 / PubMed Central
MethodsEM (single particle)
Resolution4.3 Å
Structure data

EMDB-0193, PDB-6hcg:
Klebsiella pneumoniae type II secretion system outer membrane complex. PulD, PulS and PulC HR domain.
Method: EM (single particle) / Resolution: 4.3 Å

Source
  • klebsiella pneumoniae (bacteria)
KeywordsBacterial Outer Membrane Proteins / Bacterial Proteins / Cryoelectron Microscopy / Klebsiella pneumoniae / Membrane Proteins / Microscopy, Electron / Negative Staining / PulC protein, Klebsiella pneumoniae / PulD protein, Klebsiella pneumoniae / Type II Secretion Systems / PROTEIN TRANSPORT / Type II secretion system / secretin / outer membrane channel

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