- EMDB-0193: Klebsiella pneumoniae type II secretion system outer membrane com... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-0193
タイトル
Klebsiella pneumoniae type II secretion system outer membrane complex. PulD, PulS and PulC HR domain.
マップデータ
Masked sharpened map
試料
複合体: Klebsiella pneumoniae type II secretion system outer membrane complex. PulD, PulS and PulC HR domain.
タンパク質・ペプチド: Type II secretion system protein D
タンパク質・ペプチド: Pullulanase
タンパク質・ペプチド: Pectic enzymes secretion protein OutC
キーワード
Type II secretion system / secretin / outer membrane channel / PROTEIN TRANSPORT
機能・相同性
機能・相同性情報
protein secretion by the type II secretion system / type II protein secretion system complex / intracellular protein transport / cell outer membrane / plasma membrane 類似検索 - 分子機能
Type II secretion system protein GspC / Chaperone lipoprotein, PulS/OutS / Chaperone lipoprotein, PulS/OutS-like / PulS/OutS-like superfamily / Type II secretion system pilotin lipoprotein (PulS_OutS) / Bacterial type II secretion system protein C signature. / Type II secretion system protein GspC, N-terminal / Type II secretion system protein C / Type II secretion system protein GspD / : ...Type II secretion system protein GspC / Chaperone lipoprotein, PulS/OutS / Chaperone lipoprotein, PulS/OutS-like / PulS/OutS-like superfamily / Type II secretion system pilotin lipoprotein (PulS_OutS) / Bacterial type II secretion system protein C signature. / Type II secretion system protein GspC, N-terminal / Type II secretion system protein C / Type II secretion system protein GspD / : / GspD-like, N0 domain / GspD/PilQ family / Bacterial type II secretion system protein D signature. / Type II secretion system protein GspD, conserved site / : / NolW-like / NolW-like superfamily / Bacterial type II/III secretion system short domain / Type II/III secretion system / Bacterial type II and III secretion system protein / PDZ superfamily 類似検索 - ドメイン・相同性
General secretion pathway protein C / Pullulanase D protein / Pullulanase-specific type II secretion system component C / Pullulanase secretion protein PulS / General secretion pathway protein D 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2019 タイトル: Core architecture of a bacterial type II secretion system. 著者: Anastasia A Chernyatina / Harry H Low / 要旨: Bacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here we use negative stain and cryo-electron microscopy to reveal the ...Bacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here we use negative stain and cryo-electron microscopy to reveal the core architecture of an assembled T2SS from the pathogen Klebsiella pneumoniae. We show that 7 proteins form a ~2.4 MDa complex that spans the cell envelope. The outer membrane complex includes the secretin PulD, with all domains modelled, and the pilotin PulS. The inner membrane assembly platform components PulC, PulE, PulL, PulM and PulN have a relative stoichiometric ratio of 2:1:1:1:1. The PulE ATPase, PulL and PulM combine to form a flexible hexameric hub. Symmetry mismatch between the outer membrane complex and assembly platform is overcome by PulC linkers spanning the periplasm, with PulC HR domains binding independently at the secretin base. Our results show that the T2SS has a highly dynamic modular architecture, with implication for pseudo-pilus assembly and substrate loading.