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Structure paper

タイトル	Structure and Function of a Bacterial Gap Junction Analog.
ジャーナル・号・ページ	Cell, Vol. 178, Issue 2, Page 374-384.e15, Year 2019
掲載日	2019年7月11日
著者	Gregor L Weiss / Ann-Katrin Kieninger / Iris Maldener / Karl Forchhammer / Martin Pilhofer /
PubMed 要旨	Multicellular lifestyle requires cell-cell connections. In multicellular cyanobacteria, septal junctions enable molecular exchange between sister cells and are required for cellular differentiation. ...Multicellular lifestyle requires cell-cell connections. In multicellular cyanobacteria, septal junctions enable molecular exchange between sister cells and are required for cellular differentiation. The structure of septal junctions is poorly understood, and it is unknown whether they are capable of controlling intercellular communication. Here, we resolved the in situ architecture of septal junctions by electron cryotomography of cryo-focused ion beam-milled cyanobacterial filaments. Septal junctions consisted of a tube traversing the septal peptidoglycan. Each tube end comprised a FraD-containing plug, which was covered by a cytoplasmic cap. Fluorescence recovery after photobleaching showed that intercellular communication was blocked upon stress. Gating was accompanied by a reversible conformational change of the septal junction cap. We provide the mechanistic framework for a cell junction that predates eukaryotic gap junctions by a billion years. The conservation of a gated dynamic mechanism across different domains of life emphasizes the importance of controlling molecular exchange in multicellular organisms.
リンク	Cell / PubMed:31299201 / PubMed Central
手法	EM (トモグラフィー) / EM (サブトモグラム平均)
解像度	25.0 - 35.0 Å
構造データ	EMDB-4949: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 wild type filament 手法: EM (トモグラフィー) EMDB-4950: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 gfp-fraD expressing filament 手法: EM (トモグラフィー) EMDB-4951: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 SjcF1 mutant filament 手法: EM (トモグラフィー) EMDB-4952: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 SepJ mutant filament 手法: EM (トモグラフィー) EMDB-4953: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 FraD mutant filament 手法: EM (トモグラフィー) EMDB-4954: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 FraC/FraD mutant filament 手法: EM (トモグラフィー) EMDB-4955: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 FraC mutant filament 手法: EM (トモグラフィー) EMDB-4956: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 AmiC1 mutant filament 手法: EM (トモグラフィー) EMDB-4957: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 wild type filament after CCCP treatment 手法: EM (トモグラフィー) EMDB-4961: Subtomogram average of septal junctions from Anabaena sp. PCC7120 after 45s CCCP treatment 手法: EM (サブトモグラム平均) / 解像度: 35.0 Å EMDB-4962: Subtomogram average of septal junctions from Anabaena sp. PCC7120 in the closed state after CCCP treatment 手法: EM (サブトモグラム平均) / 解像度: 28.0 Å EMDB-4963: Subtomogram average of septal junctions from Anabaena sp. PCC7120 in the open state after washing out CCCP 手法: EM (サブトモグラム平均) / 解像度: 35.0 Å EMDB-4964: Subtomogram average of septal junctions from Anabaena sp. PCC7120 AmiC1 mutant 手法: EM (サブトモグラム平均) / 解像度: 35.0 Å EMDB-4965: Subtomogram average of septal junctions from Anabaena sp. PCC7120 SepJ mutant 手法: EM (サブトモグラム平均) / 解像度: 35.0 Å EMDB-4966: Subtomogram average of septal junctions from Anabaena sp. PCC7120 SjcF1 mutant 手法: EM (サブトモグラム平均) / 解像度: 35.0 Å EMDB-4967: Subtomogram average of septal junctions from Anabaena sp. PCC7120 gfp-fraD expressing mutant 手法: EM (サブトモグラム平均) / 解像度: 28.0 Å EMDB-4968: Subtomogram average of septal junctions from Anabaena sp. PCC7120 after 24h in the dark 手法: EM (サブトモグラム平均) / 解像度: 35.0 Å EMDB-4969: Subtomogram average of septal junctions from Anabaena sp. PCC7120 in the open state 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å
由来	Nostoc sp. PCC 7120 (バクテリア)