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TitleRemoval of type IV pili by a small RNA virus.
Journal, issue, pagesScience, Vol. 384, Issue 6691, Page eadl0635, Year 2024
Publish dateApr 5, 2024
AuthorsJirapat Thongchol / Zihao Yu / Laith Harb / Yiruo Lin / Matthias Koch / Matthew Theodore / Utkarsh Narsaria / Joshua Shaevitz / Zemer Gitai / Yinghao Wu / Junjie Zhang / Lanying Zeng /
PubMed AbstractThe retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen . The single-stranded RNA (ssRNA) phage PP7 binds to T4P and is brought to the cell surface through ...The retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen . The single-stranded RNA (ssRNA) phage PP7 binds to T4P and is brought to the cell surface through pilus retraction. Using fluorescence microscopy, we discovered that PP7 detaches T4P, which impairs cell motility and restricts the pathogen's virulence. Using cryo-electron microscopy, mutagenesis, optical trapping, and Langevin dynamics simulation, we resolved the structure of PP7, T4P, and the PP7/T4P complex and showed that T4P detachment is driven by the affinity between the phage maturation protein and its bound pilin, plus the pilus retraction force and speed, and pilus bending. Pilus detachment may be widespread among other ssRNA phages and their retractile pilus systems and offers new prospects for antibacterial prophylaxis and therapeutics.
External linksScience / PubMed:38574145
MethodsEM (single particle)
Resolution3.6 - 7.9 Å
Structure data

41625

8tum

EMDB-41625, PDB-8tum:
Type IV pilus from Pseudomonas PAO1 strain
Method: EM (single particle) / Resolution: 3.6 Å

41632

8tux

EMDB-41632: Asymmetric reconstruction of mature PP7 virions
PDB-8tux: Capsid of mature PP7 virion with 3'end region of PP7 genomic RNA
Method: EM (single particle) / Resolution: 3.9 Å

41633

8tuw

EMDB-41633, PDB-8tuw:
Type IV pilus from Pseudomonas PAO1 strain with PP7 Maturation protein
Method: EM (single particle) / Resolution: 7.9 Å

EMDB-41675: The original consensus map of PP7 binding to T4P from PAO1
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-41780: Composite map of PP7 binding to type IV pilus from PAO1
Method: EM (single particle) / Resolution: 3.9 Å

Source
  • Pseudomonas aeruginosa (bacteria)
  • pseudomonas aeruginosa pao1 (bacteria)
  • pseudomonas phage pp7 (virus)
KeywordsMEMBRANE PROTEIN / Type IV pilus / T4P / PAO1 / VIRAL PROTEIN / Maturation protein / PP7 / pepevirus

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