[English] 日本語
EMN Papers
- Database of articles cited by 3DEM data entries -

+
Search query

Keywords
Author
Journal
IF

-
Structure paper

TitleStructure of the human Mediator-bound transcription preinitiation complex.
Journal, issue, pagesScience, Vol. 372, Issue 6537, Page 52-56, Year 2021
Publish dateApr 2, 2021
AuthorsR Abdella / A Talyzina / S Chen / C J Inouye / R Tjian / Y He /
PubMed AbstractEukaryotic transcription requires the assembly of a multisubunit preinitiation complex (PIC) composed of RNA polymerase II (Pol II) and the general transcription factors. The coactivator Mediator is ...Eukaryotic transcription requires the assembly of a multisubunit preinitiation complex (PIC) composed of RNA polymerase II (Pol II) and the general transcription factors. The coactivator Mediator is recruited by transcription factors, facilitates the assembly of the PIC, and stimulates phosphorylation of the Pol II C-terminal domain (CTD) by the TFIIH subunit CDK7. Here, we present the cryo-electron microscopy structure of the human Mediator-bound PIC at a resolution below 4 angstroms. Transcription factor binding sites within Mediator are primarily flexibly tethered to the tail module. CDK7 is stabilized by multiple contacts with Mediator. Two binding sites exist for the Pol II CTD, one between the head and middle modules of Mediator and the other in the active site of CDK7, providing structural evidence for Pol II CTD phosphorylation within the Mediator-bound PIC.
External linksScience / PubMed:33707221 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 7.1 Å
Structure data

23255

7lbm

EMDB-23255, PDB-7lbm:
Structure of the human Mediator-bound transcription pre-initiation complex
Method: EM (single particle) / Resolution: 4.8 Å

EMDB-23256:
Focused refinement on the cPIC within the human Mediator-bound transcription pre-initiation complex
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-23257:
Focused refinement of cTFIIH within the human Mediator-bound transcription pre-initiation complex
Method: EM (single particle) / Resolution: 7.1 Å

EMDB-23258:
Focused refinement on the Mediator Head module within the human Mediator-bound transcription pre-initiation complex
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-23259:
Focused refinement of the Mediator Middle module and TFIIH CAK module within the human Mediator-bound transcription pre-initiation complex
Method: EM (single particle) / Resolution: 6.5 Å

EMDB-23260:
Focused refinement on the Med14 C-terminus within the human Mediator-bound transcription pre-initiation complex
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-23261:
Focused refinement on the Mediator Tail module within the human Mediator-bound transcription pre-initiation complex
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-23262:
Focused refinement on Med1 within the human Mediator-bound transcription pre-initiation complex
Method: EM (single particle) / Resolution: 5.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

Source
  • homo sapiens (human)
  • Human (human)
KeywordsTRANSCRIPTION/DNA / pre-initiation complex / Mediator / initiation / complex / transcription / TRANSCRIPTION-DNA complex

+
About EMN Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Oct 5, 2021. Nobel Prize for mechanically activated and temperature-gated ion channels

Nobel Prize for mechanically activated and temperature-gated ion channels

  • The Nobel Prize in Physiology or Medicine 2021 was awarded jointly to David Julius and Ardem Patapoutian "for their discoveries of receptors for temperature and touch."
  • EM Navigator can help to find cryo-EM structure data by both pioneers.

External links:The Nobel Prize in Physiology or Medicine 2021 - NobelPrize.org / Structure data by Ardem Patapoutian / Structure data by David Julius

+
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jul 5, 2019. Downlodablable text data

Downlodablable text data

Some data of EM Navigator services can be downloaded as text file. Software such as Excel can load the data files.

PageDataFormat
EMN Searchsearch resultCSV, TSV, or JSON
EMN statisticsdata tableCSV or TSV

Related info.:EMN Search / EMN Statistics

-
EMN Papers

Database of articles cited by 3DEM data entries

  • Database of articles cited by 3DEM data entries in EMDB and PDB
  • Using PubMed data

Related info.:EMDB / PDB / Q: What are the data sources of EM Navigator? / EM Navigator / Yorodumi Papers / Changes in new EM Navigator and Yorodumi

Read more